AptaTaq Fast DNA Polymerase by Merck: Achieve High-Speed, High-Specificity PCR for Diverse Templates
Discover unparalleled efficiency in PCR workflows with AptaTaq Fast DNA Polymerase by Merck. Designed for researchers who demand rapid results without compromising specificity, this advanced enzyme meets the challenges of modern molecular biology, enabling faster block cycling and consistent success across various templates, including high GC-content DNA (up to 66% GC).
Benefits and Key Features of AptaTaq Fast DNA Polymerase
- Faster PCR completion: Completes end-point PCR reactions faster, helping shorten total PCR workflow time.
- High specificity: Supports high-specificity PCR performance for more reliable amplification outcomes.
- Reduced optimization effort: Reduces the need for assay optimization while maintaining fast end-point PCR performance.
- Broad template compatibility: Successfully amplifies a wide variety of templates with different GC content, including GC-rich targets up to 66% GC content, using a single standardized protocol.
- Convenient workflow handling: Allows PCR reaction setup at room temperature instead of on ice, simplifying day-to-day workflow management.
- Ready-to-use format: Supplied in a ready-to-use format suitable for fast PCR block cycling.
- Immediate activation: Provides immediate enzyme activation from the start of thermal cycling.
- Functionally tested: Function tested using block cycler and qPCR to verify performance.
Applications and Use Cases of AptaTaq Fast DNA Polymerase
- Fast PCR and block cycling: Intended for fast PCR protocols, including fast PCR block cycling.
- End-point PCR reactions: Suitable for end-point PCR with extension times as short as 4 minutes, without extensive assay optimization.
- Template range: Supports amplification of a large variety of templates with different GC content, including fragments with up to 66% GC content, using one protocol.
- PCR setup conditions: Enables PCR reaction setup at room temperature instead of on ice.
AptaTaq Fast DNA Polymerase Selection
| Taq DNA Polymerase | Enzyme + Buffer | |
|---|---|
| Concentration | Pack Size |
| GMP Grade, 5 U/µl | 1,000 U for up to 2,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 500 U for up to 1,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 2,500 U (10 × 250 U) for up to 5,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 5,000 U (20 × 250 U) for up to 10,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 1 U/µl | 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| Taq DNA Polymerase, dNTPack | Enzyme + Buffer + dNTP Set | |
| 5 U/µl | 100 U for up to 200 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 500 U (2 × 250 U) for up to 1,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 2,500 U (10 × 250 U) for up to 5,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 5 U/µl | 5,000 U (20 × 250 U) for up to 10,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 1 U/µl | 250 U for up to 500 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| 1 U/µl | 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 µl final volume, each containing 0.5 U Taq DNA Polymerase |
| PCR Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs | |
| – | 1 kit for up to 200 PCR reactions of 50 µl final reaction volume, each containing 2.5 U Taq DNA Polymerase |
Chemical Express, with over 23 years of expertise, is the official distributor of Merck in Thailand, offering AptaTaq Fast DNA Polymerase and related PCR reagents and accessories. Trust Chemical Express for seamless ordering and local support for Merck’s high-performance molecular biology solutions in Thailand.
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