Ascentis® Express 120 Å C18 pH+ U/HPLC Columns by Merck—Optimal High pH Stability and Fast Chromatography for Analytical Method Development
Meeting the rigorous demands of modern analytical laboratories, Ascentis® Express 120 Å C18 pH+ U/HPLC Columns from Merck deliver exceptional stability, speed and versatility for analyses requiring high pH tolerance and high-temperature operation. Engineered with Fused-Core® particle technology, these columns enable robust separations of acids, bases, and challenging analytes—making them ideal for pharmaceutical, clinical, and research environments.
Why Choose Ascentis® Express 120 Å C18 pH+ U/HPLC Columns
- High pH and high-temperature reliability: Supports demanding separations with exceptional stability in high pH mobile phases and elevated-temperature operating conditions.
- Better results for basic compounds: Improves separations for basic analytes that can show poor peak shape, insufficient retention, or limited load tolerance at low pH.
- Broader method development flexibility: Enables robust method development across a wide pH range for selective separation of acids, bases, neutrals, and zwitterions.
- Faster, more efficient chromatography: Delivers enhanced separation speed and high chromatographic efficiency to help laboratories achieve fast analyses without sacrificing performance.
- LC-MS compatibility: Offers excellent suitability for LC-MS applications where reliable chromatographic performance is required.
- Reduced runtime and solvent consumption: Supports method modernization by reducing analysis time and significantly decreasing mobile phase use while maintaining required impurity resolution.
- Consistent long-term performance: Maintains stable retention, symmetrical peak shapes, and stable back pressure during extended high pH use.
- Reliable reproducibility: Provides strong lot-to-lot consistency, supporting method validation with low retention time variability across multiple compounds.
Key Design Features
- Fused-Core® particle technology: Ascentis® Express 120 Å C18 pH+ U/HPLC Columns are built on the Fused-Core® particle technology platform.
- Particle platform: The column uses superficially porous particles (SPP).
- Alkaline-resistant surface design: It features surface-modified organo-silane technology for alkaline resistance.
- Silica composition: The stationary phase is based on Type B, high purity silica.
- Phase chemistry: The column chemistry is dimethyloctadecylsilane with a surface-modified C18 phase.
- Endcapping: The phase is endcapped.
- USP classification: Ascentis Express 120 Å C18 pH+ HPLC Columns are designated as USP L1.
Technical Specifications
- Silica: Type B (High purity silica)
- Particle platform: Superficially porous particles (SPP)
- Phase chemistry: Dimethyloctadecylsilane, surface modified
- Endcapped: Yes
- USP: L1
- Particle size: 2.7 μm
- Pore size: 120 Å
- Carbon load: 5.6%
- Surface area: 75 m²/g
- pH range: 2-12
- Maximum temperature: 60 °C
- Pressure stability: 600 bar
Available Column Formats in the Ascentis® Express 120 Å C18 pH+ Range
| Length | I.D. (mm) | Description |
|---|---|---|
| 50 mm | 1.5 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 5 cm x 1.5 mm I.D. |
| 100 mm | 1.5 | Ascentis® Express 120 Å C18 pH+, 2.7 μm, 10 cm x 1.5 mm I.D. |
| 150 mm | 1.5 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 15 cm x 1.5 mm I.D. |
| 50 mm | 2.1 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 5 cm x 2.1 mm I.D. |
| 100 mm | 2.1 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 10 cm x 2.1 mm I.D. |
| 150 mm | 2.1 | Ascentis® Express 120 Å C18 pH+, 2.7 μm, 15 cm x 2.1 mm I.D. |
| 50 mm | 3.0 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 5 cm x 3.0 mm I.D. |
| 100 mm | 3.0 | Ascentis® Express 120 Å C18 pH+, 2.7 μm, 10 cm x 3.0 mm I.D. |
| 150 mm | 3.0 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 15 cm x 3.0 mm I.D. |
| 50 mm | 4.6 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 5 cm x 4.6 mm I.D. |
| 100 mm | 4.6 | Ascentis® Express 120 Å C18 pH+, 2.7 μm, 10 cm x 4.6 mm I.D. |
| 150 mm | 4.6 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 15 cm x 4.6 mm I.D. |
| 250 mm | 4.6 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, 25 cm x 4.6 mm I.D. |
| 5 mm | 2.1 | Ascentis® Express 120 Å C18 pH+, 2.7 µm, Guard column 5 mm x 2.1 mm I.D. |
| 5 mm | 3.0 | Ascentis® Express 120 Å C18 pH+, 2.7 μm, Guard column 5 mm x 3.0 mm I.D. |
Chemical Express, with over 23 years of expertise and as the official distributor of Merck in Thailand, provides reliable access to Ascentis® Express 120 Å C18 pH+ columns and other premium analytical solutions. Enhance your chromatography workflow by ordering genuine products from the trusted experts at Chemical Express.
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