Ascentis® Express ES-Cyano HPLC Columns by Merck: Enhanced Selectivity and Efficiency for Analytical Chemistry Applications
Achieving accurate and efficient chromatographic separation is essential in analytical chemistry. The Ascentis® Express ES-Cyano HPLC column meets the industry’s demand for performance and reliability by combining superficially porous particle (SPP) technology with a unique diisopropylcyanopropyl bonded phase — delivering enhanced selectivity for both reversed-phase and mixed-mode HPLC separations. Discover how this column can optimize your separation workflows and address your laboratory’s challenges.
Key Features & Benefits of Ascentis® Express ES-Cyano HPLC Columns
- Superficially porous particle (SPP) technology: Built on a Type B high-purity silica core with a 90 Å pore size, available in 2.0 μm, 2.7 μm, and 5.0 μm particle sizes to support method development and transfer across a range of throughput and pressure requirements.
- Distinct selectivity for structurally similar compounds: The diisopropylcyanopropyl bonded phase provides differentiated retention based on hydrophobicity, shape, hydrogen-bonding capacity, and ion-exchange interactions — resolving compounds that may co-elute on conventional reversed-phase columns.
- Reduced secondary interactions for cleaner chromatograms: Endcapped construction and high-purity Type B silica minimize unwanted silanol activity, supporting sharper peaks and more reproducible retention times.
- Broad operational pH flexibility: Rated for use between pH 1 and pH 8, accommodating a wide variety of mobile phase conditions without compromising column integrity.
- Reliable performance under elevated temperature conditions: Tolerates up to 80 °C at low pH (40 °C at high pH), enabling temperature-assisted separations that improve peak shape and reduce analysis time.
Technical Specifications of Ascentis® Express ES-Cyano HPLC Columns
| Parameter | Value |
|---|---|
| Silica | Type B (High purity silica) |
| Particle Platform | Superficially porous particles (SPP) |
| Phase Chemistry | Diisopropylcyanopropyl |
| Particle Size | 2.0 μm / 2.7 μm / 5.0 μm |
| Pore Size | 90 Å |
| Carbon Load | 3.4% / 3.5% / 2.5% |
| Surface Area | 120 m²/g / 135 m²/g / 90 m²/g |
| pH Range | 1 – 8 |
| Max Temperature at Low pH | 80 °C |
| Max Temperature at High pH | 40 °C |
| Endcapped | Yes |
Applications of Ascentis® Express ES-Cyano HPLC Columns
- Reversed-phase separations: Suitable for hydrophobicity-based separations requiring differential retention of nonpolar compounds.
- Shape-selective separations: Resolves structurally similar compounds, including planar and non-planar isomers, where conventional reversed-phase columns may be insufficient.
- Hydrogen-bonding interaction separations: Suited for analytes with varying hydrogen-bonding capacities, such as phenolic compounds and polar aromatics.
- Mixed-mode separations with ion-exchange contribution: Effective for basic compounds such as amines, where the stationary phase’s acidic ion-exchange capacity enhances retention and selectivity.
- Acidic mobile phase HPLC methods: Compatible with aqueous/organic systems containing acidic modifiers such as phosphoric acid with acetonitrile, suitable for UV-detection workflows.
With over 23 years of expertise, Chemical Express is the official Merck distributor in Thailand, supplying the Ascentis® Express ES-Cyano and the full range of Merck HPLC columns to laboratories across the country.
Contact our technical team for product recommendations, method development support, or to place an order directly through Chemical Express.
Get Expert Advice or Order Ascentis® Express ES-Cyano Today
Simply fill out our Inquiry Form or chat with us instantly on Line using the buttons below.
We’re here to help!
English 
Thai