Ascentis® Express PCS-C18, 2.7 µm U/HPLC Columns by Supelco: Optimized Separation of Basic, Acidic, and Neutral Compounds

Achieving precise separation of basic, acidic, and neutral compounds is crucial in analytical chemistry. The Ascentis® Express PCS-C18, 2.7 µm U/HPLC Columns from Supelco feature innovative Positive Charged C18 Chemistry and advanced Fused-Core technology, delivering superior peak shapes and resolution. Discover how this column can transform your small molecule analyses and pharmaceutical method development.

Why Choose Ascentis®Express PCS-C18, 2.7 µm U/HPLC Columns

• Reliable separation across analyte classes: Supports effective separation of basic, acidic, or neutral compounds under low ionic strength mobile phase conditions using formic acid.
• Better results for basic compounds: Provides improved peak shape and increased loading capacity for basic compounds compared to traditional C18 chemistries.
• Higher throughput without sacrificing quality: Enables fast separations with maximum resolution for small molecule analysis in pharmaceutical method development.
• Instrument workflow flexibility: Offers excellent compatibility with both UHPLC and LC-MS methods.
• Stronger performance for challenging analyses: Enhances separation performance for peptide mapping and for basic pharmaceutical compounds prone to peak tailing.
• Demonstrated comparative advantage: Outperforms a leading competitor SPP column by delivering superior resolution, improved tailing factors, and increased plate count.

Key Features and Chemistry

• Column chemistry: Ascentis Express PCS-C18 2.7 µm U/HPLC Columns use Positive Charged C18 Chemistry based on a positively charged surface (PCS) stationary phase.
• Phase design: The stationary phase contains dimethyloctadecylsilane together with a positively charged ligand.
• Particle technology: These columns are built on advanced Fused-Core particles and are described in the specifications as superficially porous particles.
• Particle structure: The particle design incorporates 90 Å pores for the column packing structure.
• Design intent: The columns are designed for effective separation of basic, acidic, or neutral compounds under low ionic strength mobile phase conditions using formic acid.
• Small molecule analysis context: The 90 Å Fused-Core particle format is identified as suitable for small molecule analysis in pharmaceutical method development.
• Analytical context: The positively charged surface stationary phase is particularly suited to the analysis of basic analytes when low ionic strength mobile phases such as formic acid are used.
• Platform compatibility: The product is presented as a U/HPLC column format with UHPLC and LC-MS compatibility.

Technical Specifications

Applications and How It’s Used

• Small molecule analysis in pharmaceutical method development: The column is identified as ideal for small molecule analysis in pharmaceutical method development, using advanced Fused-Core® particles with 90 Å pores and Positive Charged C18 Chemistry.
• Analysis of basic, acidic, or neutral compounds: The Ascentis Express PCS-C18 2.7 µm U/HPLC Columns are used for effective separation of basic, acidic, or neutral compounds under low ionic strength mobile phase conditions such as formic acid.
• Use with low ionic strength mobile phases: The positively charged surface stationary phase is used with low ionic strength mobile phases such as formic acid, including conditions based on water with 0.1% formic acid and acetonitrile with 0.1% formic acid.
• Peptide mapping: The column is stated to provide enhanced separation performance for peptide mapping.
• Basic pharmaceutical compounds prone to peak tailing: It is used for basic pharmaceutical compounds prone to peak tailing, with the source specifically noting improved peak shape and increased loading capacity for basic compounds compared to traditional C18 chemistries.
• Separation of antidepressants: The column is employed to separate tricyclic antidepressants effectively, with example analytes including doxepin, desipramine, imipramine, nortriptyline, amitriptyline, and trimipramine under formic acid mobile phase conditions.
• Direct separation of mixed analyte types: The provided chromatographic examples include separation of compounds such as uracil, imipramine, 4-methoxybenzoic acid, and 2-chlorobenzoic acid using isocratic conditions, a 100 mm × 2.1 mm I.D. column format, 0.4 mL/min flow rate, and 35 °C.

Available Products in the Ascentis®Express PCS-C18, 2.7 µm U/HPLC Column Line

• Analytical columns: Available in 50, 100, and 150 mm lengths with 1.5, 2.1, 3.0, and 4.6 mm internal diameters.
• Guard columns: Available as 5 mm guard columns in 3 packs for 1.5, 2.1, 3.0, and 4.6 mm internal diameters.

Choose Chemical Express, the official distributor of Merck and Supelco in Thailand, with over 23 years of expertise, for your Ascentis® Express PCS-C18, 2.7 µm U/HPLC Columns. Access this and other premium analytical products from trusted brands, backed by our technical support and dedication to customer satisfaction.