Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm U/HPLC Columns by Supelco: Enhanced Selectivity for Aromatic and Basic Analyte Separations

Achieving precise separation of basic and aromatic compounds is crucial for reliable analytical chromatography. The Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm U/HPLC columns address these core needs with a positively charged surface, delivering alternative selectivity, sharper peaks, and increased loading capacity compared to conventional C18 columns. Discover how this column revolutionizes separations in pharmaceutical and analytical labs.

Why Choose Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm U/HPLC Columns

• Alternative selectivity versus C18: Provides an alternative selectivity compared to C18 columns for separations where a different retention profile is beneficial.
• Improved separations of basic analytes: Delivers excellent peak shape for basic compounds to support more reliable chromatographic performance.
• Higher loading capacity for basic compounds: Offers increased loading capacity for basic analytes, helping maintain performance across a wider injection range.
• Fast separations with high resolving power: Supports fast separations with maximum resolution for efficient analytical workflows.
• UHPLC and LC-MS compatibility: Provides excellent compatibility with both UHPLC and LC-MS methods.
• Better resolution for closely retained analytes: Improved peak shape and selectivity effectively resolved imipramine and desipramine, achieving baseline resolution where stated.
• Sharper peaks at low and high analyte loads: Produced sharper peaks for the imipramine and desipramine peak pair at both 20 ng and 200 ng.
• Reduced peak tailing for antidepressant analysis: Demonstrated improved performance for basic analytes by significantly reducing peak tailing in nortriptyline separations.
• Less overload in loaded injections: Showed less overload than the non-charged Phenyl-Hexyl column in nortriptyline injections from 20 ng to 200 ng.
• Well suited for aromatic pharmaceutical analytes: Recommended for separating aromatic compounds such as small molecule pharmaceuticals, including analytes with both basic and aromatic functional groups.

Key Features and Phase Design

• Phase design: Ascentis® Express PCS Phenyl-Hexyl 2.7 µm U/HPLC Columns use a Positive Charge Surface (PCS) Phenyl-Hexyl phase.
• Bonded chemistry: The stationary phase includes a PhenylHexyl bonded phase designed to provide an alternative selectivity relative to alkyl C18 phase chemistry.
• Interaction profile: The PhenylHexyl bonded phase supports pi-pi interactions with aromatic analytes, which can alter separation selectivity relative to the alkyl C18 phase.
• Ligand composition: The phase chemistry consists of dimethylphenyl-hexyl and a positively charged ligand.
• Charged surface concept: The column incorporates a positively charged surface through its ligand design as part of the PCS format.
• Particle technology: The column is built on superficially porous particles (FPP).
• Core-shell platform: The particle design uses Advanced Fused-Core® technology.
• Column positioning: This column is presented as an alternative to C18 columns for methods where Phenyl-Hexyl selectivity is of interest.

Technical Specifications

• Silica: Type B (High purity silica)
• Particle platform: Superficially porous particles (FPP)
• Phase chemistry: Dimethylphenyl-hexyl and positively charged ligand
• USP: L11
• Particle size: 2.7 μm
• Pore size: 90 A
• Carbon load: 6.10%
• Surface Area: 125 m²/g
• pH range: 2-7
• Max temperature: 60 °C
• Endcapped: Yes

Applications and Example Uses

• Recommended separations: Suitable for separating aromatic compounds such as small molecule pharmaceuticals, including compounds that contain both basic and aromatic functional groups.
• Pharmaceutical impurity analysis: Used for isocratic separation of imipramine and its N-demethylation product, desipramine, as shown in the impurity analysis example.
• Antidepressant analysis: Applied to the separation of antidepressants, with nortriptyline shown as an example analyte at 20 to 200 ng.
• LC workflows: Applicable in UHPLC and LC-MS workflows based on the stated compatibility of the column.

Available Products in the Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm Line

The Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm product line is available in multiple column lengths and internal diameters, including guard column formats shown under the ordering information.

• Analytical column formats: Length options include 50, 100, and 150 mm across 1.5, 2.1, 3.0, and 4.6 mm I.D.
• Guard column formats (3 pk): Guard columns are listed at 5 mm length for 2.1, 3.0, and 4.6 mm I.D.

With more than 23 years of expertise, Chemical Express is the official distributor of Merck and Supelco in Thailand. Easily purchase Ascentis® Express PCS Phenyl-Hexyl and related chromatography products through Chemical Express for trusted quality and excellent service.