Ascentis® Express Peptide ES-C18 by Merck: Advanced HPLC Columns for Superior Peptide and Protein Separation

Ascentis® Express Peptide ES-C18 by Merck: Advanced HPLC Columns for Superior Peptide and Protein Separation

Peptide and protein separations demand innovation, precision, and durability. Ascentis® Express Peptide ES-C18 columns address these industry challenges with advanced Fused-Core® particles and larger pores designed for high molecular weight compounds, ensuring exceptional resolution and robust performance for pharmaceutical and research laboratories. This page explores its advantages and key applications for analytical chromatography.

Benefits of Ascentis® Express Peptide ES-C18

  • Higher peak capacity: Provides greater resolution for peptide and small protein separations.
  • Exceptional ruggedness: Supports a long column lifetime in routine analytical use.
  • Low pH and elevated temperature tolerance: Handles demanding acidic and heated operating conditions well.
  • Expanded application range: Supports analysis of higher molecular weight compounds including peptides and small proteins.

Features of Ascentis® Express Peptide ES-C18

  • Target analyte architecture: Engineered for separation of higher molecular weight compounds such as peptides and small proteins.
  • Particle technology: Uses Fused-Core® particles.
  • Pore design: Incorporates larger pores than standard Ascentis® Express columns.
  • Bonded phase chemistry: Uses a steric-protected C18 bonded-phase.
  • Surface attachment chemistry: The C18 chain is attached to the surface through a siloxane bond with extremely high resistance to acid-catalyzed hydrolysis.

Technical Specifications of Ascentis® Express Peptide ES-C18

Ascentis® Express Peptide ES-C18 Specifications
Silica:Type B (High purity silica)
Particle Platform:Superficially porous particles (SPP)
Phase Chemistry:Diisobutyloctadecyl
Particle Size:2.7 µm
Pore Size:160 Å
Carbon Load:4.6%
Surface Area:90 m²/g
pH Range:1 – 8
Max Temperature at Low pH:90 °C
Max Temperature at High pH:40 °C
Endcapped:No

Application and Usage of Ascentis® Express Peptide ES-C18

  • Pharmaceutical and therapeutic peptide separation: Used for separation of peptide compounds in pharmaceutical and therapeutic workflows.
  • Peptide mapping: Used for peptide mapping applications in analytical chromatography.
  • Natural and synthetic peptide analysis: Used for analysis of both natural and synthetic peptides.
  • Oligonucleotide analysis: Used for analytical separation and analysis of oligonucleotides.

With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand. Discover and order Ascentis® Express Peptide ES-C18 columns and other high-quality products from Merck through Chemical Express for your analytical needs.

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