Ascentis® Express RP-Amide HPLC Columns by Merck – Enhanced Selectivity and Improved Peak Shape for Basic Compounds
Achieving optimal separation of basic and polar compounds is a common challenge in analytical chromatography. The Ascentis® Express RP-Amide HPLC columns from Merck offers improved selectivity, better peak shapes for basic analytes, and compatibility with 100% aqueous and LC-MS workflows. This page outlines how the RP-Amide phase addresses these separation challenges in modern laboratories.
Benefits of Ascentis® Express RP-Amide
- Improved peak shape for basic compounds supports cleaner separations in reversed-phase analysis.
- Increased selectivity for polar compounds, particularly hydrogen bond donors, expands separation options for challenging analytes.
- 100% aqueous compatibility allows use in fully aqueous mobile phase conditions.
- LC-MS compatibility supports integration into mass spectrometry-based analytical workflows.
- Fast separation helps laboratories complete analyses more efficiently.
- Baseline resolution of related catechols and resorcinols demonstrates stronger phenolic separations than a C18 phase in the cited example.
- Substitution for C18 without changing the mobile phase can help resolve co-eluting peaks during method development.
Features of Ascentis® Express RP-Amide
- U/HPLC column format.
- RP-Amide phase architecture providing an alternative reversed-phase selectivity to C18.
- One-step RP-Amide bonding chemistry.
- Fused-Core® technology platform.
Technical Specifications of Ascentis® Express RP-Amide
| Ascentis® Express RP-Amide Specifications | |
|---|---|
| Silica: | Type B (High purity silica) |
| Particle Platform: | Superficially porous particles (SPP) |
| Phase Chemistry: | Amide-C16 |
| Particle Size: | 2.0 µm / 2.7 µm / 5.0 µm |
| Pore Size: | 90 Å |
| Carbon Load: | 7.3% / 8.3% / 5.1% |
| Surface Area: | 120 m²/g / 135 m²/g / 90 m²/g |
| pH Range: | 2 – 9 |
| Max Temperature at Low pH: | 60 °C |
| Max Temperature at High pH: | 40 °C |
| Endcapped: | Yes |
Application and Usage of Ascentis® Express RP-Amide
- Separation of basic and polar compounds in analytical chromatography.
- Use in U/HPLC workflows.
- Operation in 100% aqueous workflows.
- Use in LC-MS workflows.
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