Benzonase® Family from Merck: Efficient DNA Removal for Vaccine & Gene Therapy Processes

Biopharmaceutical manufacturing demands efficient DNA removal solutions that ensure both reliability and compliance. The Benzonase® Family, trusted for over 30 years, delivers high-quality endonucleases to support vaccine and gene therapy production from harvest through downstream processing. Discover how these advanced enzymes meet complex industry challenges by reducing DNA contaminants and improving process consistency.

Benefits of The Benzonase® Family

• Efficient DNA removal: Reduces DNA levels in biopharmaceutical production and supports cleaner processing streams.
• Reliable process consistency: Provides reliable and consistent performance trusted in biopharmaceutical production for over 30 years.
• Compliance support: Helps balance process efficiency with regulatory compliance in manufacturing environments.
• Broader downstream process support: Supports vaccine and gene therapy processes from harvest throughout the entire downstream process.
• Lower process viscosity: Decreases viscosity during processing to support downstream operations.
• Downstream equipment protection: Helps protect downstream equipment from DNA fouling.
• Purification suitability: Serves as a suitable tool for purification of viral vaccines, viral vectors for vaccine, cell and gene therapy, and oncolytic applications.

Features of The Benzonase® Family

• Portfolio role: Smart solution portfolio for DNA removal in biopharmaceutical production.
• Enzyme classification: Benzonase® products are endonucleases.
• Manufacturing standard: Production under excipient GMP.
• Biological origin: Benzonase® endonuclease originates from the bacterium Serratia marcescens.
• Expression system: Benzonase® endonuclease is expressed in E. coli K12.
• Nuclease profile: Nonspecific activity against nucleic acids including DNA, RNA, circular, single-stranded, and double-stranded forms.
• Protease profile: No detectable proteolytic activity.
• Salt tolerant variant identity: Benzonase® Salt Tolerant endonuclease Emprove® Expert is a newly developed enzyme within the portfolio.
• Sequence distinction: Benzonase® Salt Tolerant endonuclease Emprove® Expert differs in amino acid sequence from other Benzonase® endonucleases.
• Engineering basis: Benzonase® Salt Tolerant endonuclease Emprove® Expert was created using protein engineering capabilities with intent for high activity at high salt concentrations.

Technical Specifications of Benzonase® Salt Tolerant endonuclease Emprove® Expert

• Protein form: Monomer
• Molecular weight: About 27 kDa
• Isoelectric point: pI 9.68
• Functional pH range: pH 6 to 10
• Functional temperature range: 0 °C to 37 °C
• Mg²⁺ requirement: 1 to 10 mM

Applications and Usage of The Benzonase® Family

• Vaccine processes: Used in vaccine manufacturing processes from harvest throughout the entire downstream process.
• Gene therapy processes: Used in gene therapy manufacturing processes from harvest throughout the entire downstream process.
• Viral vaccine purification: Used as a tool for the purification of viral vaccines.
• Viral vector purification: Used for purification of viral vectors for vaccine, cell and gene therapy.
• Oncolytic applications: Used in purification workflows for oncolytic applications.

Product range of Benzonase® Family

With over 23 years of industry expertise, Chemical Express is the official distributor of Merck in Thailand, offering the Benzonase® Family and other trusted products. Access high-quality solutions for your vaccine and gene therapy manufacturing needs through Chemical Express today.