BIOshell™ A400 Protein U/HPLC Columns by Merck: Superior Protein Separation for mAbs, ADCs, and Biosimilars

Protein characterization demands precise, efficient separations, especially for mAbs, ADCs, and biosimilars. BIOshell™ A400 Protein U/HPLC Columns meet these rigorous requirements with a 0.2 µm porous shell for rapid, high-resolution results, outperforming traditional fully porous particles. Discover how your lab can benefit from enhanced throughput and reliable, temperature-stable chromatography in protein analysis.

Key Benefits of BIOshell™ A400 Protein U/HPLC Columns

• Rapid and efficient protein separations: Delivers faster separations without sacrificing resolution, enabling higher throughput in protein characterization workflows.
• Narrower peak widths and improved resolution: Outperforms fully porous particle (FPP)-packed columns by producing superior peak shape and efficiency for large proteins.
• Reliable performance in high-temperature acidic conditions: Tolerates temperatures up to 90°C in acidic mobile phases, expanding the range of usable chromatographic conditions.
• Minimal signal interference in LC-MS analysis: Produces minimal to no column bleed, ensuring cleaner mass spectrometry data for confident biomolecule characterization.
• Broad instrument compatibility: Integrates seamlessly into existing UHPLC, HPLC, and MS setups without requiring additional system modifications.

Features of BIOshell™ A400 Protein U/HPLC Columns

• Porous shell architecture: A 0.2 µm-thick porous shell with 400 Å pores forms the outer layer of each particle, enabling rapid mass transfer during protein separations.
• Superficially porous particle (SPP) design: Particles consist of a solid core surrounded by a porous outer shell, distinguishing them structurally from fully porous particles (FPPs).
• Temperature stability: Columns are engineered to withstand operating temperatures up to 90°C in acidic mobile phases.
• Multi-system compatibility: Columns are compatible with UHPLC, HPLC, and mass spectrometry (MS) instrumentation.
• Low LC-MS bleed: Column construction results in minimal to no bleed, making them suitable for direct coupling with mass spectrometry detection.
• BIOshell™ A400 Protein C4 phase chemistry: Features a dimethylbutylsilane ligand (USP designation L26) on a 3.4 µm particle with 400 Å pores.
• BIOshell™ A400 Protein C18 phase chemistry: Features a diisobutyloctadecylsilane ligand (USP designation L1) on a 3.4 µm particle with 400 Å pores.

Technical Specifications of BIOshell™ A400 Protein U/HPLC Columns

Applications of BIOshell™ A400 Protein U/HPLC Columns

• Monoclonal Antibodies (mAbs): Used for the chromatographic separation and characterization of intact monoclonal antibodies.
• Antibody-Drug Conjugates (ADCs): Applied in the separation and analysis of ADCs, which require high-resolution methods due to their structural complexity.
• Biosimilars: Supports the characterization of biosimilar molecules, where precise resolution is critical for comparability assessments.
• Proteins: Suitable for the general separation and analysis of a broad range of protein samples.
• mAb Fragments: Used for the separation of monoclonal antibody fragments, enabling detailed structural characterization of these smaller biomolecular species.

Trust Chemical Express, the official distributor of Merck in Thailand with more than 23 years of expertise, for your BIOshell™ A400 Protein U/HPLC Columns and other analytical solutions. Explore our comprehensive portfolio of Merck products, ensuring you access high-performance chromatography tools and expert support for all your laboratory needs.