BIOshell™ Glycan U/HPLC Columns: Superior Glycan Separation for Analytical HPLC Applications

Achieving precise glycan profiling by HPLC remains a major challenge in analytical chemistry, especially for complex oligosaccharide separations. BIOshell™ Glycan U/HPLC Columns leverage Fused Core® Superficially Porous Silica Particle technology to deliver high-speed, high-resolution HILIC glycan analysis of released and labeled glycans — including procainamide-labeled biopharmaceutical glycans. This page details how BIOshell™ Glycan U/HPLC Columns meet demanding industry requirements for glycan profiling quality, USP L95 compliance, and analytical productivity.

Benefits of BIOshell™ Glycan U/HPLC Columns

• Higher Speed and Efficiency: Delivers greater speed and efficiency than fully porous particles of the same size, enabling faster analytical workflows without sacrificing performance.
• Fast HPLC Upgrade Without New Equipment: Converts any existing HPLC system into a fast HPLC workhorse, maximizing speed and performance without requiring a full instrument replacement.
• High-Resolution Glycan Profiling: Provides precise resolution of oligosaccharides, including both released and labeled glycans, supporting accurate and reproducible glycan analysis.
• Enhanced Retention of Challenging Analytes: Offers increased retention of acidic and zwitterionic analytes compared to bare silica columns, broadening the range of glycan species that can be effectively analyzed.
• USP L95 Method Compatibility: Supports compliance with USP L95 methods, making it suitable for regulated analytical environments and standardized testing protocols.
• Consistent Lot-to-Lot Quality: Every lot is quality control tested using a defined series of labeled glycans spanning 2 to 25 glucose units, ensuring reliable and reproducible performance across batches.

Features of BIOshell™ Glycan U/HPLC Columns

• Phase Chemistry: Proprietary penta-hydroxy chemistry tethered to the particle surface as the stationary phase, enabling robust HILIC retention of polar glycan species.
• Core Technology: Fused Core® Superficially Porous Silica Particle (SPP) technology delivers the narrow particle-size distribution and reduced band broadening that underpin the column’s speed and efficiency advantages.
• Non-Endcapped Design: The open surface chemistry maximises polar interactions with acidic and zwitterionic analytes, complementing the HILIC retention mechanism.

Technical Specifications of BIOshell™ Glycan U/HPLC Columns

Application of BIOshell™ Glycan U/HPLC Columns

• Oligosaccharide Resolution via HILIC: The primary application is the separation of released and labeled glycans using hydrophilic interaction liquid chromatography (HILIC), making it well-suited for glycan profiling workflows.
• Biopharmaceutical Glycan Analysis: Supports HPLC analysis of procainamide-labeled human IgG glycans using a HILIC-FLR (fluorescence detection) method — a standard approach in biopharmaceutical glycan profiling workflows. Typical operating conditions:
  • Column format: 150 mm × 2.1 mm I.D., 2.7 μm
  • Mobile phase additive: 50 mM ammonium formate (pH 4.4)
  • Detection: Fluorescence (FLR)

With over 23 years of expertise in scientific and analytical chemistry distribution, Chemical Express is the official distributor of Merck in Thailand. Contact Chemical Express to order BIOshell™ Glycan U/HPLC Columns and receive expert support tailored to your chromatography and glycan analysis workflows.