CellPrime® rTrypsin Recombinant Trypsin by Merck: High-Purity Cell Dissociation for Stem Cell Culture, Vaccine & Bioprocessing

Ensuring the integrity of adherent cells during harvest is vital for vaccine production, stem cell research, and bioprocessing. CellPrime® rTrypsin by Merck offers a high-purity, non-animal origin solution to efficiently dissociate and resuspend cells while preserving crucial surface markers and process consistency across scales.

Benefits & Key Features of CellPrime® rTrypsin

• Reliable cell harvest support: Enables efficient dissociation and resuspension of adherent cells during harvest, supporting robust vaccine production workflows.
• Cell surface marker preservation: Helps dislodge adherent cells in adult human stem cell culture while preserving the structural and functional integrity of cell surface markers.
• Bioprocessing relevance: Supports therapeutic recombinant insulin manufacturing by facilitating pro-insulin to active recombinant insulin maturation.
• Non-animal origin profile: Eliminates animal-derived components, helping reduce variability and regulatory concerns in cell culture and bioprocessing workflows.
• Broad process fit: Suitable for use across Master Cell Bank (MCB), Working Cell Bank (WCB), production raw materials, manufacturing processes, and final product workflows.
• Versatile cell culture utility: Suitable for a broad range of life science cell culture applications beyond bioprocessing.
• Recombinant expression: Recombinantly expressed in the yeast Pichia pastoris using a synthetic DNA construct encoding the porcine trypsin gene sequence for consistent activity.
• Flexible formats: Available as lyophilized powder and as a ready-to-use liquid formulation, in multiple pack sizes.

Technical Specifications

• Expression system: Recombinantly expressed by a synthetic DNA construct that encodes the porcine gene sequence in the yeast Pichia pastoris.
• Origin classification: Non-animal origin product and does not contain components of animal origin.
• Applicable process stages: Suitable for use in Master Cell Bank (MCB) and Working Cell Bank (WCB) preparation, as well as in production raw materials, manufacturing processes, and final product workflows.
• Activity note: rTrypsin activity determined according to USP38 (89), 2015.
• Unit equivalence: One unit of trypsin activity using carbobenzoxy-valyl-glycyl-arginine4-nitril-anilide acetate (Chromzym TRY) as the substrate corresponds to 21 USP trypsin (BAEE) units.

Applications and Use Cases

• Vaccine production: Dissociation and resuspension of adherent cells during harvest.
• Therapeutic recombinant insulin manufacturing: Pro-insulin to active recombinant insulin maturation.
• Adult human stem cell culture: Dislodging adherent cells such as hMSC while preserving the structural and functional integrity of cell surface markers.
• Stem cell cultures on carriers: Dislodging adherent cells grown on carriers with preservation of surface markers.
• General life science cell culturing: General dissociation and passaging in research and process development cell culture workflows.

CellPrime® rTrypsin Product Options

With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand. Access CellPrime® rTrypsin and other Merck products through us for trusted supply, technical support, and efficient procurement for all your cell culture and bioprocessing needs.