Enzymatic Deglycosylation Kit by Merck: Efficient Glycoprotein Analysis for Research and MS Applications

Biochemical research and MS analysis often require precise and efficient glycoprotein deglycosylation. The Enzymatic Deglycosylation Kit from Merck provides an integrated solution for laboratories, enabling effective removal of complex O-linked carbohydrates to support rigorous protein studies.

Benefits of the Enzymatic Deglycosylation Kit for Glycoprotein Analysis

• Comprehensive deglycosylation support: Enables efficient removal of O-linked carbohydrates from glycoproteins as part of broader glycan trimming workflows for dependable downstream analysis.
• Research and MS suitability: Provides a practical choice for protein studies or MS analysis where glycoprotein deglycosylation is required.
• Defined sample handling: Accommodates solutions containing up to 200 mg of glycoprotein per reaction for straightforward sample planning.
• Multi-reaction convenience: Supplies a minimum of 10 reactions, supporting repeated or parallel experimental work from one kit.

Key Features of the Enzymatic Deglycosylation Kit for Glycoprotein Analysis

• Enzyme Components: PNGase F, O-Glycosidase, α2(3,6,8,9)-Neuraminidase, β1,4-Galactosidase, and β-N-Acetylglucosaminidase.
• Buffer Component: Reaction Buffer is included as part of the kit composition.
• Preparation Reagent: Denaturation Solution is provided for sample preparation steps within the kit workflow.
• Detergent Component: Triton X-100 Solution is included among the kit reagents.
• Kit Structure: The formulation combines glycosidases and supporting solutions within one deglycosylation kit configuration.

Technical Specifications of the Enzymatic Deglycosylation Kit for Glycoprotein Analysis

• Product Number: EDEGLY
• Recommended Sample Size: Solutions containing up to 200 mg of glycoprotein.
• Sample Scale: Minimum of 10 reactions.
• Storage Temperature: 4 °C
• Shelf Life of Unused Product: Minimum of 1 year

Applications and Usage of the Enzymatic Deglycosylation Kit

• Glycoprotein deglycosylation: Used for enzymatic removal of O-linked carbohydrates from glycoproteins in preparation for downstream analytical workflows.
• Protein studies: Applied in protein research workflows where deglycosylation is required for analysis.
• MS analysis: Used to prepare glycoprotein samples for mass spectrometry analysis.

Components of the Enzymatic Deglycosylation Kit

• PNGase F, 20 µl, 0.3 unit (P2619)
• O-Glycosidase, 20 µl (G1163)
• α-2(3,6,8,9)-Neuraminidase, 20 µl (N8271)
• Fetuin Control, 10 mg/ml solution, 0.5 mg (F4301)
• 5X Reaction Buffer, 0.2 ml (R2651)
• Denaturation Solution, 0.1 ml (D6439)
• Triton X-100, 15% Solution, 0.1 ml (T3319)
• β-1,4-Galactosidase, 20 µl (G0413)
• β-N-Acetylglucosaminidase, 20 µl (A6805)

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