Expand Long Range dNTPack by Roche: High-Yield Long PCR for Genomic DNA Applications

Expand Long Range dNTPack by Roche: High-Yield Long PCR for Genomic DNA Applications

Accurately amplifying long DNA fragments can be a challenge for standard PCR systems. Expand Long Range dNTPack by Roche meets this need, delivering reliable PCR amplification for fragments from 5 to 25 kb from genomic DNA. Find out how this advanced kit solves the complexities of long-template PCR with ease.

Why Choose Expand Long Range dNTPack for Long PCR

  • Consistent long-range amplification: Supports consistent amplification of PCR products from 5 to 25 kb from genomic DNA.
  • High-quality PCR results: Delivers high specificity, yield, and increased fidelity for long-template amplification.
  • Target-focused output: Enables high yields of only the desired fragment across long-template applications.
  • Workflow efficiency: Ready-to-use enzyme, buffer, and dNTP combination reduces pipetting steps and simplifies long PCR setup.
  • Cost-effective value: Supports up to 125 reactions per pack, helping control costs in long-range PCR workflows.

 

Key Features of Expand Long Range dNTPack

  • Kit Configuration: Enzyme, buffer, and dNTP set.
  • Buffer Format: One buffer is used for all fragment sizes.
  • Reaction adjustment components: DMSO and MgCl2 solution included for reaction optimization.
  • Nucleotide Composition: Premixed PCR-Grade nucleotides are included.

 

Technical Specifications of Expand Long Range dNTPack

  • Amplification size range: 5 to 25 kb from genomic DNA
  • Pack size: 175 U
  • Reaction capacity: Up to 125 reactions
  • Final reaction volume: 20 μl
  • Catalog number: 04 829 034 001

 

Applications and Intended Use of Expand Long Range dNTPack

  • Long-range PCR: Long-range PCR assays requiring reliable amplification of 5 to 25 kb fragments from genomic DNA.
  • Amplification from genomic DNA: Used for amplification of PCR products of 5 to 25 kb from genomic DNA.
  • Long-template applications: Long-template PCR workflows such as structural variant analysis, large genomic loci characterization, or cloning of extended inserts.
  • Fragments greater than 20 kb: For targets beyond the 5 to 25 kb range covered by Expand Long Range dNTPack, use the Expand 20 kbPLUS PCR System.

 

Products Under Expand Long Range dNTPPack

ProductPack Size
Expand Long Range, dNTPack
Enzyme + Buffer + dNTP Set
175 U for up to 125 reactions of 20 µl final volume, each containing 1.4 U enzyme blend
Expand Long Range, dNTPack
Enzyme + Buffer + dNTP Set
700 U for up to 500 reactions of 20 µl final volume, each containing 1.4 U enzyme blend
Expand Long Range, dNTPack
Enzyme + Buffer + dNTP Set
3,500 U (5 × 700 U) for up to 2,500 reactions of 20 µl final volume, each containing 1.4 U enzyme blend
Expand 20 kbPLUS PCR System
Enzyme + Buffer
200 U for up to 40 reactions of 50 µl final volume, each containing 5 U enzyme blend

With more than 23 years of expertise, Chemical Express is the official distributor of Merck and Roche in Thailand. Rely on us to provide Expand Long Range dNTPack and related long PCR products, ensuring quality results for your molecular biology applications.

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