Fluorescence Indicators Merck for Analytical Chemistry: Accurate Endpoint Detection and Microscopy Applications
Laboratories require precise methods for endpoint detection and sample visualization. Fluorescence indicators such as Acridine orange zinc chloride double salt, Certistain® Calcein, Chromotropic acid disodium salt dihydrate, and others deliver accuracy for analytical titrations and enable detailed imaging of cells, chromosomes, bacteria, and antigen-antibody reactions. Discover how these high-quality indicators meet complex analytical requirements.
Benefits of Fluorescence Indicators
- Reliable endpoint determination: Supports accurate endpoint determination in precipitation titration at the equivalence point.
- Clear analytical decision making: Provides a well defined change at the equivalence point to help laboratories identify titration endpoints.
- Microscopy workflow support: Enables staining and visualization of cells, cell components, chromosomes, and bacteria.
- Immunological detection capability: Supports detection of antigen-antibody reactions.
- Broad laboratory utility: Serves both analytical chemistry requirements and microscopy based visualization tasks within one product category.
Features of Fluorescence Indicators
- Fluorescent response in solution: Fluorescence indicators are compounds or substances whose fluorescent properties in solution are influenced by changes in hydrogen-ion concentration, oxidation potential, or metal-ion concentration.
- Indicator class: These products belong to the fluorescence indicator category, defined by a change in fluorescent properties under specified chemical conditions.
- Chemically responsive behavior: Their characteristic feature is the dependence of fluorescence on solution conditions related to acidity, redox state, or metal-ion concentration.
Applications and Usage of Fluorescence Indicators
Endpoint Determination in Precipitation Titration: Fluorescence indicators identify the endpoint in a precipitation titration, marking where a well-defined change occurs at the equivalence point. In this workflow, adding the reagent causes the analyte to form a hardly soluble precipitate.
Laboratory Staining and Visualization: Fluorescent dyes are widely used in laboratory work to successfully stain and visualize whole cells, specific cell components, chromosomes, and bacteria.
Antigen-Antibody Reaction Detection: These indicators are also utilized to detect specific antigen-antibody reactions.
Fluorescence Indicators Product List
| Product | Transition range (from) | Transition range (to) | Color change (from) | Color change (to) | Preparation of indicator solution |
|---|---|---|---|---|---|
| Acridine orange zinc chloride double salt (C.I.46005) for microscopy Certistain® | 8.4 | 10.4 | no fluorescence | yellow-green | 0.1 g in 100 mL Ethanol (96%) |
| Calcein indicator for metal determination | 8.0 | 10.4 | no fluorescence | yellow-green | |
| Chromotropic acid disodium salt dihydrate for analysis ACS, Reag. Ph Eur | 3.1 | 4.4 | no fluorescence | light-blue | 5.0 g in 100 mL water |
| 2′,7′-Dichlorofluorescein indicator ACS, Reag. Ph Eur | 4.0 | 6.6 | blue-green | no fluorescence | 0.1 g in 100 mL Ethanol (96%) |
| Eosin Y (yellowish) (C.I. 45380) for microscopy Certistain® | 0.0 | 3.0 | no fluorescence | green | 1 g in 100 mL water |
| Erythrosine B (C.I. 45430) for microscopy Certistain® | 2.5 | 4.0 | no fluorescence | light green | 0.2 g in 100 mL water |
| Fluorescein sodium (C.I. 45350) indicator Reag. Ph Eur | 4.0 | 4.5 | rose | green | 1.0 g in 100 mL water |
| Fluorescent indicator F254 | – | – | fluorescent green | colorless | |
| 1,2-Phenylenediamine GR for analysis | 3.1 | 4.4 | green | no fluorescence | 0.2 g in 100 mL Ethanol (96%) |
| Phloxin B (C.I. 45410) for microscopy Certistain® | 2.5 | 4.0 | no fluorescence | light green | 0.1 g in 100 mL Ethanol (96%) |
| Uranine AP (C.I. 45350) concentrated for the examination of subterranean waters | – | – |
With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand, providing access to premium fluorescence indicators such as Acridine orange zinc chloride double salt, Certistain® Calcein, and more for analytical and bioanalytical needs. Explore and purchase Merck’s high-quality products and other leading brands conveniently through Chemical Express.
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