HistoDenz™ by Sigma-Aldrich: Advanced Nonionic Density Gradient Media for Precise Cell Separation in Tissue Diagnostics

Efficient cell separation and tissue diagnostics require media with high density, low viscosity, and minimal toxicity. HistoDenz™ density gradient media meets these demanding standards, offering a nonionic, stable, and inert iodinated gradient medium designed to minimize dehydration and maximize cell viability for superior research and clinical outcomes.

Why Choose HistoDenz™ Density Gradient Media

• Reduced particle dehydration: Minimizes the dehydration of biological particles during density gradient work.
• Low toxicity profile: Offers very low toxicity for applications involving biological samples and viable cells.
• Lower osmotic burden: Low osmolality supports density gradient separations where gentler conditions are important.
• High density capability: High density enables effective use in both rate-zonal and isopycnic centrifugation.
• Lower viscosity handling: Low viscosity compared to sucrose supports density gradient workflows that require an alternative to more viscous media.
• Broad separation range: Suitable for separating everything from macromolecules to viable cells.
• Cell-compatible base compound: Iohexol is nontoxic and not metabolized by mammalian cells.

What HistoDenz™ Density Gradient Media Is

• Composition: Denser iohexol solution.
• Media Type: Nonionic iodinated density gradient medium.
• Compound Class: Non-ionic X-ray contrast compound developed specifically as a density gradient medium.
• Related Compound Context: Shares a number of characteristics with the related compound metrizamide.
• Solubility Profile: Soluble in water, yielding a clear, colorless solution, and also soluble in formamide and dimethylformamide.

HistoDenz™ Density Gradient Media Technical Specifications

• Catalog number: D2158
• CAS RN: 66108-95-0
• Synonym: 5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide
• Molecular formula: C19H26I3N3O9
• Molecular weight: 821.14
• λmax: 244 nm
• E^mM: 342 (H2O)
• Water solubility: 50 mg/ml, yielding a clear, colorless solution
• Maximum gradient concentration: 50% (w/v)
• Maximum density: 1.4 g/ml

Solution Properties of Histodenz

Applications and Usage of HistoDenz™ Density Gradient Media

• Density gradient separations: HistoDenz™ density gradient media is used for separating materials ranging from macromolecules to viable cells in both rate-zonal and isopycnic centrifugation.
• Non-aqueous denaturing gradients: Non-aqueous denaturing gradients up to 50% (w/v) can be prepared for rate zonal and isopycnic separations.
• Human lymphocyte isolation (example protocol): For isolation of human lymphocytes, a 14.1% (w/v) HistoDenz™ solution containing 0.44% (w/v) NaCl and 5 mM tricine at pH 7.2 is prepared and the procedure is performed at room temperature.
• Layering and centrifugation workflow: An equal volume of anticoagulated blood is mixed with 0.9% physiological saline, 6 ml of the diluted blood is carefully layered over 3 ml of the 14.1% HistoDenz™ solution, and the tube is centrifuged at 800 × g for 25 minutes at room temperature in a swingout rotor.
• Interface collection: Lymphocytes form a white layer at the interface between the plasma and the HistoDenz™ solution, and this interface layer is carefully collected with a plastic pipette.
• Optional concentration step: Collected lymphocytes may be concentrated by dilution with physiological saline followed by centrifugation at 400 × g for 10 minutes.
• Post-separation removal from samples: HistoDenz™ may be removed from samples by recentrifugation in appropriate buffer, dialysis, ultrafiltration, gel filtration, or sample precipitation with TCA or ethanol.

Chemical Express, with over 23 years of expertise, is Thailand’s official distributor of Merck and Sigma-Aldrich products. Access HistoDenz™ and a comprehensive range of quality cell separation and diagnostic solutions through Chemical Express for reliable service and professional support.