IROA TruQuant IQQ Workflow Kit by IROA Technologies – Accurate Quantitation for Mass Spectrometry Metabolomics

Accurate metabolite quantitation is crucial for reliable results in mass spectrometry-based metabolomics. The IROA TruQuant IQQ Workflow Kit is a comprehensive LC-MS metabolomics workflow solution that addresses common issues such as artifacts, noise, and chromatographic fluctuations by employing well-characterized isotopic standards and dedicated analysis software.

Benefits of the IROA TruQuant IQQ Workflow Kit

• Accurate Biological Measurements: Enables simultaneous quantitation of several hundred biochemicals from small quantities of biological samples.
• Reduced False Discovery: Removes artifacts and noise to eliminate false discovery during quantitation.
• Suppression-Corrected Data Output: Provides suppression-corrected values for more consistent quantitative assessment.
• Normalized Results: Delivers normalized data for downstream interpretation of metabolomics experiments.
• Instrument Performance Monitoring: Supports quality control by accounting for chromatography fluctuations, mass or retention time drift, and source or instrumentation malfunctions.

Key Features of the IROA TruQuant IQQ Workflow Kit

• Quantitation System Architecture: High-quality quantitation system for mass spectrometry metabolomics applications.
• Internal Standard Composition: Includes lyophilized Internal Standard identified as U-¹³C, 95% labeled.
• Long-Term Reference Standard Composition: Includes lyophilized Long-Term Reference Standard composed of paired U-¹³C, 95% labeled and 5% labeled material mixed 1:1.
• Internal Standard Scope: Provides a complex internal standard containing 600+ compounds.
• Software Component: Includes ClusterFinder software for building libraries, identifying and quantitating compounds, and normalizing generated data.
• Reference Library Content: ClusterFinder includes a library of peaks in the Long-Term Reference Standard and their physical characteristics.
• Kit Throughput Scope: Contains the materials and tools for the analysis of 90 experimental samples.

Technical Specifications for the IROA TruQuant IQQ Workflow Kit

• Storage Temperature: -70 °C
• Experimental Sample Capacity: 90 samples
• Internal Standard Vial Count: 3 vials
• Long-Term Reference Standard Vial Count: 3 vials
• Internal Standard Labeling: U-¹³C, 95% labeled
• Long-Term Reference Standard Labeling: Paired U-¹³C, 95% labeled and 5% labeled
• Long-Term Reference Standard Mix Ratio: 1:1
• Internal Standard Complexity: 600+ compounds
• Internal Standard Suitability per Amber Vial: 30 samples

Applications and Usage of the IROA TruQuant IQQ Workflow Kit in LC-MS Metabolomics

• LC-MS Metabolomics Workflows: Designed for LC-MS-based metabolomics studies using the IROA TruQuant IQQ Workflow Kit.
• Experimental Sample Analysis: For analysis of experimental samples spiked with the Internal Standard.
• Reference Standard Analysis: Long-Term Reference Standard aliquots are analyzed together with experimental samples to build a dictionary of retention time, m/z, and physical characteristics, including fragmentation data.
• Compound Identification and Targeted Analysis: ClusterFinder is used to upload Long-Term Reference Standard LC-MS data files, build a targeted library for each method or LC-MS mode, and then process experimental LC-MS data using this curated library.
• Data Export Options: Resultant dictionaries can be exported in CSV or CEF format, and targeted analysis results can be exported as TSV files.
• Injection Standard Usage: The Internal Standard may be used as an injection standard to align chromatographic peaks across runs.
• Method Development Support: The Long-Term Reference Standard may be used with alternating scans dedicated to secondary compound identification information, such as fragmentation or ion mobility.

Trust Chemical Express, with over 23 years of experience, as the official distributor of IROA Technologies and Merck in Thailand. Purchase the IROA TruQuant IQQ Workflow Kit and explore a comprehensive range of related products for advanced, reliable results through Chemical Express.