LiChroprep® Sorbent by Merck: High-Selective Silica Chromatography Resins for Reproducible Peptide and API Purification
Purifying peptides and APIs with speed, efficiency and reproducibility is crucial in pharma and biopharma manufacturing. LiChroprep® Sorbents by Merck provide excellent selectivity and are described as a very efficient and economic alternative for almost all preparative chromatography purification challenges, designed to meet the special requirements of customers’ manufacturing processes. This page details the benefits, technical features and product options of LiChroprep® RP and Si chromatography resins for peptide and API purification.
Benefits of LiChroprep® Sorbent
- Reproducible purification results: Delivers consistent, reproducible separation performance across peptide purification processes, ensuring reliable outcomes batch after batch.
- High purity output: Achieves high levels of purity in a rapid and economical manner, making it suited for purifying valuable compounds in research, pilot plant operation and production.
- Excellent selectivity and efficiency: Provides excellent selectivity and separation efficiency, enabling precise isolation of target compounds from complex mixtures.
- Cost-effective API polishing: LiChroprep® Si offers a cost-effective and reliable solution for polishing biopharmaceutical and pharmaceutical APIs without compromising quality.
- Large batch size support: Supports large batch sizes of up to 200 kg in the modification step.
- High performance and high permeability: The narrow, well-defined particle size distribution contributes to high performance and high permeability.
- Economical alternative for preparative chromatography: Irregularly shaped sorbents are a very efficient and an economic alternative for almost all preparative chromatography purification challenges.
Features of LiChroprep® Sorbent
- Silica gel matrix: Totally porous, irregularly shaped silica gel particles form the base matrix of LiChroprep® sorbents, with good separation performance and sufficient stability at reasonable cost.
- Particle size classification: Particles are tightly classified into three defined ranges: 15–25 μm, 25–40 μm, and 40–63 μm.
- Available product variants: Available as LiChroprep® RP-18, LiChroprep® RP-8, and LiChroprep® Si 60.
- Homogeneous matrix structure: The silica gel matrix is reproducible and homogeneous across production batches.
- Narrow particle size distribution: Each particle size range is characterized by a well-defined quality factor (d90/d10) to ensure consistent classification.
- Ready-to-use column format: LiChroprep® is available in prepacked, stainless steel Hibar® 250-25 mm columns for immediate use.
- Bulk pack format: Also supplied in various bulk pack sizes to support preparative and production-scale operations.
Technical Specifications of LiChroprep® Sorbent
| Parameter | LiChroprep® RP-18 15–25 μm | LiChroprep® RP-18 25–40 μm | LiChroprep® RP-18 40–63 μm | Method |
|---|---|---|---|---|
| Particle size range d50 [μm] | 18–25 | 30–35 | 55–65 | Laser diffraction |
| Quality factor d90/d10 | ≤4.0 | ≤5.0 | ≤4.0 | |
| Mean pore size [nm] | 10 | 10 | 10 | N₂ adsorption (BET) |
| Carbon content [weight %] | ≥15 | ≥15 | ≥15 | CHN analysis |
| Iron content [weight %] | ≤0.02 | ≤0.02 | ≤0.02 | |
| Specific pore volume [mL/g] | 0.95–1.03 | 0.95–1.03 | 0.95–1.03 | N₂ adsorption (BET) |
| Specific surface area [m²/g] | 330–380 | 330–380 | 330–380 | N₂ adsorption (BET) |
| Surface coverage [μmol/m²] | 3.0 | 3.0 | 3.0 | |
| Elutables [weight %] | ≤0.10 | ≤0.10 | ≤0.10 | |
| Bulk density [g/mL] | 0.39 | 0.44 | 0.48 | |
| Packing density [g/mL] | 0.51 | 0.52 | 0.60 | |
| Selectivity | ||||
| α Acetophenone/Phenol | 1.8–2.2 | 1.8–2.2 | 1.8–2.2 | |
| α Acetophenone/Cinnamic acid | 1.1–1.3 | 1.1–1.3 | 1.1–1.3 | |
| α 1-Naphthol/Phenol | 3.9–4.4 | 3.9–4.4 | 3.9–4.4 | |
| Capacity | ||||
| k’ Toluene | 17.0–22.0 | 19.0–24.0 | 17.0–22.0 |
Applications of LiChroprep® Sorbent
- Peptide purification: LiChroprep® RP sorbents are used for fast, effective, and reproducible separation in peptide purification processes.
- Biopharmaceutical and pharmaceutical API polishing: LiChroprep® Si products are well-suited for cost-effective, reliable polishing of biopharmaceutical and pharmaceutical APIs.
- Preparative chromatography for purification of valuable compounds: Used in preparative chromatography for the purification of valuable compounds in research, pilot plant operation and production.
LiChroprep® Sorbent Product Portfolio
| Description | Particle Size | Qty |
|---|---|---|
| LiChroprep® RP-18 | 15–25 μm | 0.1, 0.5, 10 kg |
| LiChroprep® RP-18 | 25–40 μm | 0.1, 0.5, 5, 25 kg |
| LiChroprep® RP-18 | 40–63 μm | 0.25, 1, 25 kg |
| LiChroprep® RP-8 | 40–63 μm | 0.25, 1 kg |
| LiChroprep® Si 60 | 15–25 μm | 1, 25 kg |
| LiChroprep® Si 60 | 25–40 μm | 1 kg |
| LiChroprep® Si 60 | 40–63 μm | 0.25, 1, 25 kg |
Trust Chemical Express, with over 23 years of expertise, as the official Merck distributor in Thailand. Access LiChroprep® Sorbent and related Merck products for preparative chromatography and purification applications. Chemical Express ensures reliable supply and expert support for your pharma and biopharma needs.
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