Sf-RVN® Insect Cell Line by Merck: Rhabdovirus-Free Platform for Safe Recombinant Protein, VLP, and AAV Production

Biosafety and regulatory compliance are critical in bioprocessing, especially for recombinant protein and viral vector production. The Sf-RVN® Insect Cell Line by Merck addresses these needs with a rhabdovirus-negative, GMP-banked cell line offering excellent growth and productivity, optimized for modern gene therapy and vaccine applications. This solution helps mitigate contamination risks while supporting robust, compliant workflows.

Why Choose Sf-RVN® Insect Cell Line

• Improved bioprocess safety profile: Enhances risk mitigation and biosafety by using a rhabdovirus-negative cell line, helping address process contaminant concerns associated with most Spodoptera frugiperda cells such as Sf-9.
• High performing production platform: Provides an optimized platform for producing recombinant proteins, viral vaccines such as virus-like particles (VLPs), and adeno-associated virus (AAV) vectors for gene therapy applications.
• Confidence for regulated workflows: Full traceability and documentation are available to support regulatory filings.
• Comparable Sf-9 cell characteristics: Delivers the same characteristics as Sf-9 cells while offering a rhabdovirus-negative profile.

Key Features of Sf-RVN® Insect Cell Line

• Cell line identity: Sf-RVN® Insect Cell Line is a rhabdovirus-negative Sf9 (Spodoptera frugiperda) cell line.
• Culture format: The cell line can be grown in both adherent and suspension culture.
• Medium adaptation: Sf-RVN® Insect Cell Line has been adapted for growth in chemically defined medium.
• Process position: The cell line is used for the production of recombinant proteins, adeno-associated viruses (AAV), and virus-like particles (VLP).
• Testing and banking: The cell line was tested for adventitious agents and banked under GMP conditions.
• Documentation: A technical user guide with detailed protocols for optimal performances is provided with the cell line.
• Platform configuration: Sf-RVN® Insect Cell Line and the recommended EX-CELL® CD Insect Cell Medium together form the Sf-RVN® Platform.
• Companion medium: EX-CELL® CD Insect Cell Medium is a chemically defined medium recommended for use with the cell line.
• Product presentation: Sf-RVN® cells are provided in vials containing 1 mL at 10 × 10⁶ cells/mL.
• Banking formulation: The cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO.

Technical Specifications of Sf-RVN® Insect Cell Line

• Vial fill volume: 1 mL
• Cell concentration: 10 × 10^6 cells/mL
• Banking medium: EX-CELL® CD Insect Cell Medium with 10% DMSO
• Typical culture viability: 95–98% when cultivated in EX-CELL® CD Insect Cell Medium with passaging every 3–4 days
• Growth assay result: Typical day 7 cell density of 8 × 10^6 cells/mL in a duplicate seven-day assay

Applications and Intended Use

• Recombinant protein production: Supports recombinant protein production in chemically defined medium.
• AAV for gene therapy: Supports production of adeno-associated viruses (AAV) for gene therapy applications.
• Virus-like particles (VLPs): Enables production of VLPs for viral vaccine applications.
• Sf-RVN® Platform: When used together with companion EX-CELL® CD Insect Cell Medium, the cell line forms a platform optimized for excellent growth and productivity in recombinant protein, AAV, and VLP production workflows.
• Intended use: The Sf-RVN® Insect Cell Line is sold for research use only, and a license agreement must be acquired before use of the cell line or any products derived from it in a clinical or commercial process.

Sf-RVN® Insect Cell Line Product List and Supporting Reagents

Chemical Express, with over 23 years of expertise, is the official distributor of Merck and the mentioned brands in Thailand. Procure Sf-RVN® Insect Cell Line and related products, ensuring reliable supply and technical support for your advanced bioprocessing needs through Chemical Express.