Stable Isotope Labeled Amino Acid Mixes from Sigma-Aldrich: Certified Reference Materials for Advanced Analytical and Mass Spectrometry Applications

Precise quantification of amino acids is crucial for pharmaceutical, biological, and analytical research applications. Stringent industry demands require accuracy, traceability, and compliance in amino acid analysis. MERCK’s Stable Isotope Labeled Amino Acid Mixes, featuring TraceCERT® certified reference materials, address these needs by providing high-quality internal standards for isotope dilution mass spectrometry and reliable calibration for LC-MS, GC-MS, and MALDI analyses. Discover how these solutions streamline your workflow and ensure SI traceability.

Benefits of Stable Isotope Labeled Amino Acid Mixes

• Confident quantification support: Certified reference materials of stable isotope labeled amino acid analogues are used as internal standards and references for isotope dilution mass spectrometry (IDMS) in amino acid analysis.
• Accuracy and bias control: Combining these certified reference materials with natural amino acids enables measurement and calibration of instrumental biases.
• SI traceability for compliance needs: TraceCERT® certified reference materials are traceable to primary material from NIST, supporting traceability to the SI.
• Fewer experiments without sacrificing traceability: Applicable to more complex techniques such as isobaric tagging (iTRAQ, aTRAQ™), helping reduce the number of experiments while still maintaining traceability to the SI.
• Broad mass spectrometry applicability: Suitable as internal standards or references for mass spectrometric analysis of amino acids by isotope dilution methods and other research applications including LC-MS, GC-MS, and MALDI.
• Documentation for defensible results: Supplied with a comprehensive certificate including measurement uncertainty to support confident reporting and method documentation.

Features of Stable Isotope Labeled Amino Acid Mixes

• Product type: MERCK Stable Isotope Labeled Amino Acid Mixes are certified solution mixtures of stable isotope labeled analogues of the corresponding amino acids, supplied under the TraceCERT® certified reference material designation.
• Portfolio context: Two new certified solution mixtures of labelled amino acids have been recently added to MERCK’s reference material portfolio of more than 20,000 products, within a broader offering of amino acid reference materials.
• Mix formats available: The two mixes are provided as Stable Isotope Labeled Amino Acid Mix Solution 1 (Product Number 96378) and Stable Isotope Labeled Amino Acid Mix Solution 2 (Product Number 01428).
• Certified reference material traceability: TraceCERT® certified reference material, traceable to primary material from NIST.
• Production framework: Isotopically Labeled Amino Acid Mix produced by an established workflow following ISO 17034.
• Component certification: qNMR certified components in the mix, following ISO/IEC 17025 accreditation.
• Homogeneity and stability testing: Tested for homogeneity and stability using an LC method.
• Documentation: Supplied with a comprehensive certificate including measurement uncertainty.
• Preparation and reporting note: Prepared with L-amino acids; the certification methods do not distinguish enantiomers, therefore the certified values are reported without specifying the stereochemistry.

Application and Usage 

• Amino acid analysis (AAA): Use the mixes in workflows where peptide bonds in a protein or peptide are hydrolyzed, followed by analysis of the released amino acids using appropriate analytical methods.
• Pharmaceutical and biological sample testing: Apply AAA to determine amino acid composition or protein content in pharmaceutical formulations and biological preparations.
• Biological fluids: Use AAA for biological fluids such as urine, blood, or plasma samples.
• Protein powder supplements: Use AAA to determine amino acid composition or protein content in protein powder supplements.
• Internal standards and references for IDMS: Use the stable isotope labeled amino acid certified reference materials as internal standards and as references for isotope dilution mass spectrometry (IDMS).
• Calibration with natural amino acids: Combine the certified stable isotope labeled analogues with the corresponding natural amino acids to enable measurement and calibration of instrumental biases.
• Mass spectrometric techniques: Use as internal standard or reference for mass spectrometric analysis of amino acids by isotope dilution methods and other research applications, including LC-MS, GC-MS, and MALDI.
• Isobaric tagging workflows: Apply the same approach in more complex techniques such as isobaric tagging (iTRAQ, aTRAQ™).

Stable Isotope Labeled Amino Acid Mix Products

• Stable Isotope Labeled Amino Acid Mix Solution 1
  • Product Number: 96378
  • Product Name: Stable Isotope Labeled Amino Acid Mix Solution 1
  • Suffix: certified reference material, TraceCERT®
  • Solvent: HCl (0.1M)
  • Concentrations: 1250-2500 nmol/mL
  • Package Size: 1 mL
  • Components (CAS, nominal concentration):
    • L-Alanine-13C3, 15N (CAS 202407-38-3): 2.5 mmol/L
    • L-Arginine-13C6 (CAS 55443-58-8): 2.5 mmol/L
    • L-Aspartic acid-13C4 (CAS 55443-54-4): 2.5 mmol/L
    • L-Cystine-13C6, 15N2 (CAS 1252803-65-8): 1.25 mmol/L
    • L-Glutamic acid-13C5 (CAS 55443-55-5): 2.5 mmol/L
    • Glycine-13C2,15N (CAS 211057-02-2): 2.5 mmol/L
    • L-Histidine-13C6 (CAS 55443-59-9): 2.5 mmol/L
    • L-Isoleucine-13C6, 15N (CAS 202468-35-7): 2.5 mmol/L
    • L-Leucine-13C6, 15N (CAS 202406-52-8): 2.5 mmol/L
    • L-Lysine13C6 (CAS 55443-57-7): 2.5 mmol/L
    • L-Methionine-13C5, 15N (CAS 202468-47-1): 2.5 mmol/L
    • L-Phenyl-13C6-alanine (CAS 180268-82-0): 2.5 mmol/L
    • L-Proline-13C5 (CAS 201740-83-2): 2.5 mmol/L
    • L-Serine-13C3, 15N (CAS 202407-34-9): 2.5 mmol/L
    • L-Threonine-13C4 (CAS 55443-53-3): 2.5 mmol/L
    • L-Tyrosine-(phenyl-13C6) (CAS 201595-63-3): 2.5 mmol/L
    • L-Valine-13C5 (CAS 55443-52-2): 2.5 mmol/L




• Stable Isotope Labeled Amino Acid Mix Solution 2
  • Product Number: 01428
  • Product Name: Stable Isotope Labeled Amino Acid Mix Solution 2
  • Suffix: certified reference material, TraceCERT
  • Solvent: HCl (0.1M)
  • Concentrations: 500-2500 nmol/mL
  • Package Size: 1 mL
  • Components (CAS, nominal concentration):
    • L-Alanine-13C3, 15N (CAS 202407-38-3): 0.5 mmol/L
    • L-Arginine-13C6 (CAS 55443-58-8): 0.5 mmol/L
    • L-Aspartic acid-13C4 (CAS 55443-54-4): 0.5 mmol/L
    • L-Glutamic acid-13C5 (CAS 55443-55-5): 0.5 mmol/L
    • Glycine-13C2, 15N (CAS 211057-02-2): 2.5 mmol/L
    • L-Leucine-13C6, 15N (CAS 202406-52-8): 0.5 mmol/L
    • L-Methionine-13C5, 15N (CAS 202468-47-1): 0.5 mmol/L
    • L-Phenyl-13C6-alanine (CAS 180268-82-0): 0.5 mmol/L
    • L-Proline-13C5 (CAS 201740-83-2): 0.5 mmol/L
    • L-Tyrosine-(phenyl-13C6) (CAS 201595-63-3): 0.5 mmol/L
    • L-Valine-13C5 (CAS 55443-52-2): 0.5 mmol/L




With over 23 years of expertise, Chemical Express is the official distributor of MERCK and Supelco in Thailand. Access a comprehensive selection of certified MERCK Stable Isotope Labeled Amino Acid Mixes and related reference materials through Chemical Express. Trust us for seamless procurement and expert support for your analytical chemistry and mass spectrometry workflows.

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