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Cell Culture

Cell culture plays an important role in biology, life sciences and pharmacological research and manufacturing. Our Cell Culture & Analysis products ensure that you have the tools needed to drive breakthroughs in cell biology, drug discovery, regenerative medicine and so on.

From basic cell culture essentials to specialized formulations, our products support a wide array of cell types and applications to meet the diverse needs of researchers and professionals. Whether you are performing routine cell counting, cell imaging, or advanced cellular assays, our products deliver unparalleled performance and consistency.

Cell Culture Media & Buffers

Whether you’re cultivating adherent or suspension phenotypes, with or without FBS, and prefer high- or low-glutamine options, we have you covered. Our ready-to-use liquid and easy-to-store powder formulations cater to your convenience. And rest assured, our media and salts are meticulously qualified for cell culture applications and manufactured in cutting-edge facilities.

  • Bioprocessing Cell Culture Media
  • Classical Media & Buffers
  • Plant Culture Media

Cell Lines & Specialty Cell Culture

Depending on your research, you’ll need the specific cell lines or primary cells of interest. Our curated selection ensures reliable and high-quality options, allowing you to advance your experiments with confidence. 

  • Stem Cells
  • Mouse Embryo Media
  • Primary Cells
  • Mammalian Cell Lines
  • Bioprocessing Catalog Cell Lines

3D Cell Culture

Designed to mimic the in vivo environment, our 3D Cell Culture products provide great physiological relevance, fostering more accurate cell behavior and responses. Our user-friendly products ensure high cell viability, enhanced physiological relevance, and accurate representation of cell behavior.

  • Hydrogels
  • Scaffolds
  • Organoids

Sterile Lab Media Filtration & Cultureware

Sterile laboratory media filters play a vital role in preventing contamination in cultures. Designed to eliminate particulate contamination, sterilize liquid reagents, and clarify solutions, our filters come in various formats like syringe filters, vacuum-driven filter units, and bottle top filters. Featuring high-performance membranes, our filtration solutions ensure the integrity of laboratory reagents, buffers, and media. 

  • Sterile Laboratory Filtration Devices
  • Vacuum Filtration
  • Filter Plates
  • Syringe Filters
  • Cell Culture Supplies
  • Cell Culture Inserts & Plates

Cell Culture Supplements & Reagents

Ensuring adequate cell growth relies on many culture conditions, including the correct media formulation for your cell line. Tailored for diverse cell types, our high-quality supplements and reagents enhance viability, proliferation, and functionality. From serum alternatives to specialized growth factors, ensure reliable results for your research and bioprocessing needs.

  • Cell Freezing Reagents
  • Plant Culture Reagents
  • Cell Dissociation Reagents
  • Albumins and Transport Proteins
  • Hormones
  • Supplements for Cell Culture
  • Sera
  • Human Serum
  • Fetal Bovine Serum (FBS)
  • Attachment Factors
  • Amino Acids
  • Antibiotics
  • Carbohydrates
  • Vitamins
  • Growth Factors and Cytokines

Cell Analysis

Various cell analysis techniques are employed to understand factors influencing cell proliferation, death, health, and function. Explore a spectrum of cell analysis techniques with our instruments and reagents. From live cell imaging to cell counting, health assessment, isolation, and migration studies, our tools empower researchers. In addition to standard kits, our specialty instruments offer extra dimensions for in-depth cell process investigations.

  • Live Cell Imaging Reagents
  • Cell Health Analysis
  • Cell Migration & Invasion Assays
  • Cell Isolation Kits
  • Cell Counting Equipment
  • Cell Imaging Instruments

Animal Component Free Cell Culture Reagents

Explore our Animal Component Free Cell Culture Reagents—ideal for researchers seeking consistent and ethical alternatives. Our reagents, free from animal-derived ingredients, ensure optimal cell growth with minimal lot-to-lot variations. Our high-quality animal component-free (ACF), non-animal origin (NAO) and serum-free cell culture media, supplements, reagents, and growth factors have high biological activity. Compliant with high-quality and sustainability standards, they’re perfect for applications like stem cell culture, inflammation studies, cancer cell culture, and antibody/vaccine development. 

  • Animal origin-free & serum-free media
  • Animal-free cell dissociation reagents
  • Chemically defined supplements
  • Animal component-free growth factors & Cytokines
  • Animal-free cell freezing media

Discover an extensive collection of Cell Culture & Analysis products we offer from Sigma Aldrich through our Search Tool.

 For other brands, please contact us.

SectionNameCat.No.
SerumFetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridomaF2442
SerumFetal Bovine Serum, Heat Inactivated, USA origin, sterile-filtered, suitable for cell cultureF4135
SerumFetal Bovine Serum, USDA approved, non-USA origin, sterile-filtered, suitable for cell cultureF0926
SerumFetal Bovine Serum, USDA approved, non-USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture12306C
SerumFetal Bovine Serum, Non-USA origin, sterile-filtered, suitable for cell cultureF7524
SerumFetal Bovine Serum, Heat Inactivated, Non-USA origin, sterile-filtered, suitable for cell cultureF9665
Amino AcidsL-Glutamine solution, 200mM, solution, sterile-filtered, suitable for cell cultureG7513
Amino AcidsMEM Non-essential Amino Acid Solution (100×), liquid, without L-glutamine, sterile-filtered, suitable for cell cultureM7145
AntibioticsAmphotericin B solution, sterile-filtered, 250 μg/mL in deionized water, suitable for cell cultureA2942
AntibioticsAmphotericin B solubilized, powder, γ-irradiated, suitable for cell cultureA9528
AntibioticsAmpicillin sodium salt, suitable for cell culture, 91.0–100.5% (titration), powderA0166
AntibioticsAntibiotic-Antimycotic Stabilized, suspension, stabilized, sterile-filtered, suitable for cell cultureA5955
AntibioticsG418 disulfate salt solution, 50mg/mL solution, sterile-filtered, suitable for cell cultureG8168
AntibioticsGelatin solution, 2% in water, tissue culture grade, sterile, Type B, suitable for cell cultureG1393
AntibioticsGentamicin solution, 50mg/mL in deionized water, liquid, sterile-filtered, suitable for cell cultureG1397
AntibioticsGentamicin solution, 10mg/mL in deionized water, liquid, sterile-filtered, suitable for cell cultureG1272
AntibioticsHygromycin B from Streptomyces hygroscopicus, powder, suitable for cell culture, suitable for insect cell cultureH3274
AntibioticsMitomycin C from Streptomyces caespitosus, powder, suitable for cell cultureM4287
AntibioticsNeomycin solution, liquid, sterile-filtered, suitable for cell cultureN1142
AntibioticsNystatin preparation, suspension, sterile; aseptically processed, suitable for cell cultureN1638
AntibioticsPenicillin-Streptomycin, 100×, liquid, sterile-filtered, suitable for cell cultureP0781
AntibioticsPenicillin-Streptomycin, 50×, liquid, stabilized, sterile-filtered, suitable for cell cultureP4458
AntibioticsPenicillin-Streptomycin, 100×, liquid, stabilized, sterile-filtered, suitable for cell cultureP4333
AntibioticsPuromycin dihydrochloride from Streptomyces alboniger, powder, ≥98% (TLC), suitable for cell cultureP8833
Attachment FactorsFibronectin from bovine plasma, solution, 1mg/mL (in 0.5M NaCl, 0.05M Tris, pH 7.5), suitable for cell culture, sterileF1141
Attachment FactorsLaminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1mg/mL in Tris buffered NaCl, sterile-filtered, suitable for cell cultureL2020
Attachment FactorsPoly-D-lysine hydrobromide, average mol wt 30,000-70,000, γ-irradiated, lyophilized powder, suitable for cell cultureP7280
Attachment FactorsPoly-D-lysine hydrobromide, mol wt 70,000-150,000, γ-irradiated, lyophilized powder, suitable for cell cultureP6407
Attachment FactorsPoly-D-lysine hydrobromide, mol wt >300,000, γ-irradiated, lyophilized powder, suitable for cell cultureP7405
Attachment FactorsPoly-L-lysine hydrobromide, mol wt 70,000-150,000, γ-irradiated, lyophilized powder, suitable for cell cultureP6282
Attachment FactorsPoly-L-lysine solution, 0.01%, mol wt 70,000-150,000, sterile-filtered, suitable for cell cultureP4707
Attachment FactorsPoly-L-lysine solution, 0.01%, mol wt 150,000-300,000, sterile-filtered, suitable for cell cultureP4832
Balanced SaltsDulbecco's Phosphate Buffered Saline, With MgCl_{2} and CaCl_{2}, liquid, sterile-filtered, suitable for cell cultureD8662
Balanced SaltsDulbecco's Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, sterile-filtered, liquid, suitable for cell cultureD8537
Balanced SaltsDulbecco's Phosphate Buffered Saline, Without calcium chloride, powder, suitable for cell cultureD5773
Balanced SaltsDulbecco's Phosphate Buffered Saline, Modified, Without calcium chloride and magnesium chloride, powder, suitable for cell cultureD5652
Balanced SaltsDulbecco's Phosphate Buffered Saline 10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell cultureD1408
Balanced SaltsDulbecco's Phosphate Buffered Saline, Modified, with 1,000mg/L glucose, sodium pyruvate, streptomycin sulfate, kanamycin monosulfate, CaCl_{2}, MgCl_{2}, liquid, sterile-filtered, suitable for cell cultureD4031
Balanced SaltsEarle's Balanced Salts, With sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureE2888
Balanced SaltsHanks' Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell cultureH6648
Balanced SaltsHanks' Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell cultureH8264
Balanced SaltsHanks' Balanced Salt solution, With sodium bicarbonate, sterile-filtered, suitable for cell cultureH9269
Balanced SaltsHanks' Balanced Salt solution, Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell cultureH9394
Balanced SaltsKrebs-Henseleit Buffer Modified, With 2000mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell cultureK3753
BiochemicalsHeparin sodium salt from porcine intestinal mucosa, Grade I-A, suitable for cell culture, activity: ≥140 USP units/mg, powderH3149
Biological BuffersHEPES solution, 1M, pH 7.0-7.6, sterile-filtered, suitable for cell cultureH0887
Biological BuffersSodium bicarbonate, powder, ≥99.5%, suitable for cell culture, suitable for insect cell cultureS5761
Biological BuffersSodium bicarbonate solution, solution (7.5%), sterile-filtered, suitable for cell cultureS8761
Biological BuffersSodium chloride solution (0.9%), sterile-filtered, suitable for cell cultureS8776
Cell DissociationCell Dissociation Solution Non-enzymatic 1×, Prepared in Hanks' Balanced Salt Solution without calcium and magnesium, sterile-filtered, suitable for cell cultureC5789
Cell DissociationCell Dissociation Solution Non-enzymatic 1×, Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, suitable for cell cultureC5914
Cell DissociationTrypsin-EDTA solution, 0.25%, 2.5g porcine trypsin and 0.2g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red, sterile-filtered, suitable for cell cultureT4049
Cell DissociationTrypsin-EDTA solution, 1×, 0.5g porcine trypsin and 0.2g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red, sterile-filtered, suitable for cell cultureT3924
Cell DissociationTrypsin-EDTA solution, 10×, 5.0g porcine trypsin and 2g EDTA • 4Na per liter of 0.9% sodium chloride, sterile-filtered, suitable for cell cultureT4174
Growth FactorsEndothelial cell growth supplement from bovine neural tissue, suitable for cell cultureE2759
Growth FactorsEpidermal Growth Factor human, recombinant, expressed in Escherichia coli, lyophilized powder, suitable for cell cultureE9644
Growth FactorsFibroblast Growth Factor-Basic human, recombinant, expressed in Escherichia coli, suitable for cell cultureF0291
Growth FactorsInsulin solution from bovine pancreas, 10mg/mL insulin in 25mM HEPES, pH 8.2, sterile-filtered, suitable for cell cultureI0516
HormonesGonadotropin from pregnant mare serum, lyophilized powder, suitable for mouse embryo cell cultureG4527
HormonesInsulin from bovine pancreas, powder, suitable for cell cultureI6634
HormonesInsulin from bovine pancreas, lyophilized powder, activity: ≥25 USP units/mg, secondary activity: 2500 units (HPLC, /100mg vial), γ-irradiated, suitable for cell cultureI1882
HormonesInsulin solution human, Chemically defined, recombinant from Saccharomyces cerevisiae, solution, sterile-filtered, suitable for cell cultureI9278
Hormones & Misc.ReagentsITS Liquid Media Supplement (100×), liquid, sterile-filtered, suitable for cell cultureI3146
Hormones & Misc.ReagentsOPI Media Supplement, Hybri-Max™, γ-irradiated, lyophilized powder, suitable for hybridomaO5003
Hormones & Misc.ReagentsRed Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridomaR7757
HybridomaHAT Media Supplement (50×) Hybri-Max™, lyophilized powder, γ-irradiated, suitable for hybridomaH0262
HybridomaHistopaque®-1077 Hybri-Max™, liquid, sterile-filtered, suitable for hybridomaH8889
LectinsConcanavalin A from Canavalia ensiformis (Jackbean), Type IV-S, lyophilized powder, aseptically processed, suitable for cell cultureC5275
LipopolysaccharidesLipopolysaccharides from Escherichia coli 0111:B4, suitable for cell culture, γ-irradiatedL4391
LipopolysaccharidesLipopolysaccharides from Escherichia coli 055:B5, suitable for cell culture, γ-irradiatedL6529
LipopolysaccharidesLipopolysaccharides from Escherichia coli 026:B6, suitable for cell culture, γ-irradiatedL2654
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell cultureD5796
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridomaD5671
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureD6429
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureD6546
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell cultureD1145
MediaDulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate. Use at 13.4 g/L., powder, suitable for cell cultureD5648
MediaDulbecco's Modified Eagle's Medium-low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureD6046
MediaDulbecco's Modified Eagle's Medium-low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureD5546
MediaDulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureD6421
MediaGlasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureG5154
MediaIscove's Modified Dulbecco's Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridomaI3390
MediaL-15 Medium (Leibovitz), Without L-glutamine., liquid, sterile-filtered, suitable for cell cultureL5520
MediaMedium 199, With Earle's salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureM4530
MediaMinimum Essential Medium Eagle, HEPES Modification, with Earle's salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureM7278
MediaMinimum Essential Medium Eagle, Joklik Modification, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureM8028
MediaMinimum Essential Medium Eagle, Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, powder, suitable for cell cultureM0518
MediaMinimum Essential Medium Eagle, With Earle's salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureM4655
MediaMinimum Essential Medium Eagle, With Earle's salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureM2279
MediaMinimum Essential Medium Eagle, With Earle's salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureM5650
MediaNutrient Mixture F-10 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureN6013
MediaRPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell cultureR8758
MediaRPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell cultureR5886
MediaRPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell cultureR0883
MediaRPMI-1640 Medium, Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell cultureR7509
Miscellaneous ReagentsSodium Hydroxide Solution, 1.0N, suitable for cell cultureS2770
Miscellaneous ReagentsSodium Pyruvate Solution, 100mM, sterile-filtered, suitable for cell cultureS8636
Miscellaneous ReagentsWater, sterile-filtered, suitable for cell cultureW3500
ReagentsAlbumin from Bovine Serum, powder, Fatty Acid-free, suitable for cell culture, ≥96% (agarose gel electrophoresis)A8806
ReagentsCell Freezing Medium - DMSO 1×, sterile-filtered, suitable for cell cultureC6164
Reagents & BiochemicalsLinoleic Acid - Albumin from Bovine Serum Albumin, liquid, sterile-filtered, suitable for cell cultureL9530
Reagents & HybridomaDimethyl Sulfoxide, liquid (temperature-dependent), ≥99.7%, Hybri-Max™, sterile-filtered, suitable for hybridomaD2650
Reagents & HybridomaDimethyl Sulfoxide, Biotechnology Performance Certified, sterile-filtered, meets EP, USP testing specifications, suitable for hybridomaD2438
Stains, Dyes & IndicatorsThiazolyl Blue Tetrazolium Bromide, powder, suitable for cell culture, suitable for insect cell cultureM5655
Stains, Dyes & IndicatorsTrypan Blue Solution, 0.4%, liquid, sterile-filtered, suitable for cell cultureT8154
SupplementsD-(+)-Glucose Solution, 45% in water, tissue culture grade, autoclaved, suitable for cell cultureG8769
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