CellPrime® rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance

Achieving reliable, high-performing cell cultures with CellPrime® rTransferrin AF requires eliminating variability and contamination risks from animal-derived proteins. CellPrime® rTransferrin AF delivers consistent iron transport and robust cell growth in serum-free media, and is designed to meet demanding industry standards. This non-animal, recombinant alternative supports optimal performance and facilitates regulatory compliance.

Benefits of CellPrime® rTransferrin AF for Serum-Free Cell Culture

Benefits & Key Features of CellPrime® rTransferrin AF

• Reduced variability in media formulations: Replacing serum transferrin with this recombinant supplement helps remove variability introduced by blood-derived proteins.
• Lower contamination risk: Safeguards cell culture processes against adventitious virus or prion contamination associated with blood-derived proteins.
• Optimized serum-free performance: Promotes viability and proliferation in cells grown in serum-free media by supporting optimal iron metabolism.
• Comparable biological performance to serum transferrin: Several studies demonstrate similar potency in supporting cell growth and protein production.
• Advantage over chemical iron supplements: Provides biological, not chemical, iron supplementation and has shown superior performance compared to chemical supplements across multiple cell lines.
• Proven suitability for industrial cell culture: Recombinant transferrin expressed in Saccharomyces cerevisiae, specifically manufactured for industrial cell culture applications and used across multiple industrial cell lines.
• Reliable lot-to-lot consistency: Consistently meets product specifications from batch to batch, supporting dependable process performance.
• Regulatory-friendly, non-animal origin transferrin: Offers the advantages of plasma-derived transferrin while providing a non-animal origin, regulatory-friendly alternative for industrial cell culture applications.
• cGMP manufacturing and convenient format: Manufactured under cGMP in FDA-inspected facilities and supplied as a filtered liquid that can be directly added to culture medium, often in combination with recombinant albumin and recombinant insulin in media formulations.

Features of CellPrime® rTransferrin AF

• Product Classification: Recombinant iron-transport protein (rTransferrin) expressed as a non-animal origin (NAO) analog to plasma-derived transferrin.
• Expression System: Protein synthesized in Saccharomyces cerevisiae utilizing a recombinant yeast platform.
• Manufacturing Standard: cGMP-compliant production within FDA-inspected industrial facilities.
• Functional Mechanism: Biochemical framework designed for extracellular iron binding and transport within cell culture systems.
• Supplementation Format: Biological iron delivery architecture utilized in combination with recombinant albumin and insulin media supplements.
• Physical State: Sterility-filtered liquid formulation configured for direct integration into culture media.
• Regulatory Profile: Non-animal origin composition structured for alignment with industrial bioprocessing compliance standards.

Technical Specifications of CellPrime® rTransferrin AF

• Protein by Kjeldahl: 18-22 g/L
• Purity by RP-HPLC: ≥95%
• pH (1% solution) by standard method: 6.0-8.0
• Inspection: Clear, red/pink solution
• Endotoxin by LAL: ≤20 EU/mL
• Iron content by atomic absorption: 1.3-1.7 mg/g
• Total iron capacity by atomic absorption: ≥ 1.0 mg/g

Application and Usage of CellPrime® rTransferrin AF

• Serum-free cell culture use: CellPrime® rTransferrin AF is used in cell culture systems to facilitate extracellular iron binding and transport, supporting iron metabolism in cells grown in serum-free media.
• Media formulation component: CellPrime® rTransferrin AF is often used to replace serum transferrin in combination with recombinant albumin and recombinant insulin as a supplement mix in cell culture media formulations.
• Industrial cell culture applications: Suitable for use in upstream industrial cell culture processes across multiple cell lines.
• Direct medium addition: CellPrime® rTransferrin AF is supplied as a 0.2 μm filtered liquid formulated in 145 mM NaCl, pH 6.8, at 20 g/L and can be directly added to the culture medium.
• Recommended use concentration: A titration should be performed for each application because the optimum concentration may vary by cell type, culture conditions, and medium composition, with a recommended final concentration range of 1 to 10 mg/L.
• Substitution in existing workflows: CellPrime® rTransferrin AF can be directly substituted for bovine or human-derived transferrin.
• Filtration guidance: If additional filtration is required in your process, use a low protein-binding membrane such as polyvinylidene difluoride, polyethersulfone, or cellulose acetate with a pore size of 0.22 μm.
• Adaptation consideration: Cultures previously maintained in media containing iron chelators may require an adaptation phase, and the full effects of CellPrime® rTransferrin AF may not be apparent until after one or more sub-culturing steps.

Products Available Under CellPrime® rTransferrin AF

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