Millicell® Inserts & Plates by Merck: Advanced Cell Culture Solutions for Reliable Analysis

 

Achieving physiologically relevant cell culture models is essential for accurate research and analysis. Millicell® Inserts and Plates by Merck deliver high-performance permeable supports, enabling access to both apical and basolateral surfaces for enhanced cellular assays, making Millicell inserts and plates a reliable choice for advanced cell culture workflows in demanding cell biology and cell culture applications.

Why Choose Millicell® Inserts and Plates

• More physiologically relevant culture conditions: Provides cell access to media and nutrients from both the apical and basolateral sides, more closely mimicking the in vivo cellular environment than conventional seeding on polystyrene plates.
• Reliable access for analysis: Supports study of both apical and basal surfaces of cell monolayers for more complete cellular evaluation.
• Reduced contamination and disruption risk: Helps decrease the risk of contamination and monolayer disruption during handling.
• Improved membrane protection during pipetting: Reduces pipette-related damage to the membrane during routine cell culture work.
• Clear visualization for image-based workflows: Supports brightfield imaging, fluorescence microscopy, and fluorescent imaging for direct cell observation.
• Enhanced nutrient diffusion: Allows increased rates of basolateral diffusion of nutrients to cells.
• Efficient, automation-ready workflows: Integrates cell growth, feeding, and analysis in one membrane-bottom plate to streamline high-throughput and routine workflows.
• Broad workflow integration: Supports use with both manual and automated systems.

Key Features and Design Elements

• Permeable Support Architecture: Membrane-based culture supports provide access to media and nutrients from the apical and basolateral sides.
• Product Format Range: Millicell inserts and plates include hanging cell culture inserts, standing cell culture inserts, organotypic standing inserts, and membrane-bottom plate assemblies.
• Hanging Insert Configuration: Hanging cell culture inserts are available in 6, 12, and 24-well sizes.
• Hanging Insert Membrane Material: Hanging inserts use tissue-culture treated PET membranes in optically clear and translucent formats.
• Hanging Insert Pore Range: PET membrane pore sizes span from 0.4 µm to 8.0 µm.
• Apical Assist Rim: Hanging inserts incorporate an apical assist rim as part of the insert structure.
• Plate Compatibility: Hanging cell culture inserts are compatible with most cell culture plates.
• Sterile Packaging Format: Hanging inserts are individually blister packed and gamma sterilized.
• Fluorophore Compatibility: Hanging inserts are compatible with fluorophores for fluorescent imaging.
• Standing Insert Configuration: Standing cell culture inserts are free-standing inserts that rest at the bottom of wells and are elevated by feet.
• Standing Insert Membrane Options: Standing inserts are available with Biopore™ PTFE, MF-Millipore™ MCE, and Isopore™ PCF membranes.
• Biopore™ PTFE Membrane: Biopore™ PTFE is a low protein-binding and transparent membrane with low background fluorescence.
• MF-Millipore™ MCE Membrane: MF-Millipore™ MCE is a Triton®-free membrane option.
• Isopore™ PCF Membrane: Isopore™ PCF is a hydrophilic, tissue culture-treated membrane with high pore density.
• Organotypic Insert Profile: Organotypic standing inserts have a shorter profile that fits standard petri dishes.
• Organotypic Membrane Material: Organotypic standing inserts use an optically clear Biopore™ membrane.
• Plate Assembly Architecture: Millicell®-24 and Millicell®-96 plate assemblies are membrane-bottom plates that combine cell growth, feeding, and analysis in one plate format.
• System compatibility: Millicell plate assemblies integrate into common plate-handling and imaging systems used in cell culture labs.

Technical Specifications

• Well formats: 6 well, 12 well, and 24 well.
• Membrane pore size range: 0.4 µm to 8.0 µm.
• Hanging insert membrane material: PET.
• Hanging insert membrane options: Optically clear and translucent.
• Standing insert membrane options: Biopore™ PTFE, MF-Millipore™ MCE, and Isopore™ PCF.
• Plate assembly formats: Millicell®-24 and Millicell®-96.

Applications and Usage of Millicell inserts and plates

• Apical and basolateral cell studies: Millicell inserts and plates provide permeable support for cell growth so cells can access media and nutrients from both the apical and basolateral sides, enabling studies of both surfaces of cell monolayers.
• Co-culture: Hanging cell culture inserts are suitable for co-culture applications where cells need to be maintained without disturbance from insert feet.
• 2.5D cell culture: Hanging cell culture inserts are used for 2.5D cell culture to support cellular assays in a format that more closely mimics the in vivo cellular environment than conventional seeding on polystyrene plates.
• Chemotaxis assays: Hanging cell culture inserts are designed for chemotaxis studies, including Boyden chamber–style chemotaxis workflows.
• Migration and invasion assays: Millicell cell culture inserts support cell migration and invasion assays, including matrix-coated Boyden chamber formats.
• Organotypic cell culture: Organotypic standing inserts are designed to support 3D explant structures and organotypic cultures in standard petri dishes.
• Barrier assays: Hanging cell culture inserts are used for barrier assays, including direct growth, staining, and imaging of cells in cell culture plates.
• Fluorescent imaging: Millicell hanging cell culture inserts are compatible with fluorophores for fluorescent imaging, and the platform supports fluorescence imaging directly in cell culture plates.
• Cell growth, feeding, and analysis in one plate format: Millicell plate assemblies support streamlined assay workflows by combining cell growth, media exchange, and endpoint analysis within a single membrane-bottom plate.

Millicell® Inserts and Plates Product List

Chemical Express, with over 23 years of expertise, is the official distributor of Merck in Thailand. Researchers can purchase Millicell® Inserts, Plates, and other trusted Merck products directly through Chemical Express, ensuring genuine quality and expert support for all advanced cell culture needs.

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