Native Enzymatic Deglycosylation Kit by Merck – Efficient Glycoprotein Research, MS Analysis and Native State Studies

Efficient glycoprotein analysis is vital for reliable, reproducible research outcomes. The Native Enzymatic Deglycosylation Kit addresses common challenges in glycoprotein deglycosylation, enabling selective removal of N-linked oligosaccharides while preserving native protein conformation. This page details the product features, benefits, and applications relevant for researchers and scientists working with glycoprotein samples.

Benefits of the Native Enzymatic Deglycosylation Kit for Glycoprotein Analysis

• Support for native state studies: Helps enable glycoprotein research where preservation of the native state is important.
• MS analysis suitability: Provides a practical option for workflows focused on mass spectrometry analysis.

Technical Specifications of the Native Enzymatic Deglycosylation Kit

• Recommended Sample Size: Solutions containing up to 200 mg of glycoprotein
• Sample Scale: Minimum of 10 reactions.
• Storage Temperature: 4 °C
• Shelf Life of Unused Product: Minimum of 1 year.

Applications and Usage of the Native Enzymatic Deglycosylation Kit

• Removal of N-linked oligosaccharides: Used for enzymatic deglycosylation of glycoproteins while maintaining native conditions.
• MS analysis: Applied in workflows where native deglycosylation is required prior to mass spectrometry analysis.
• Native state studies: Suitable for studies that require glycoproteins to remain in their native conformation during deglycosylation.
• Glycoprotein research: Intended for glycoprotein analysis and research involving solutions containing up to 200 mg of glycoprotein.

Components of the Native Enzymatic Deglycosylation Kit 

• Endoglycosidase F1, 0.3 unit (E9762)
• Endoglycosidase F2, 0.1 unit (E0639)
• Endoglycosidase F3, 0.1 unit (E2264)
• Endoglycosidase F1 Reaction Buffer, 200 ml (R9025)
• Endoglycosidase F2 and F3 Reaction Buffer, 200 ml (R9150)

Trust Chemical Express, with 23 years of expertise as Merck’s official distributor in Thailand, to supply the Native Enzymatic Deglycosylation Kit and related glycoprotein research products reliably and efficiently.
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