Protein A Purification Resins and Buffers by Merck: High-Throughput Solutions for Antibody Capture
Meeting the demand for high yield and cost-effective purification, Merck’s Products for Purification Processes: Protein A are expertly designed for rapid, large-scale isolation of monoclonal, polyclonal, and engineered antibodies. Discover how these solutions support downstream processing and productivity in biopharmaceutical manufacturing.
Why choose Merck Products for Purification Processes: Protein A
- Rapid target isolation: Supports quick isolation of the target molecule from clarified feedstock during the capture phase.
- Manageable process volume: Helps reduce process volume to a manageable scale for subsequent steps.
- Yield and cost-efficiency focus: Supports purification strategies centered on maximizing yield and cost efficiency.
- High-throughput purification: Enables high-throughput purification for antibody capture workflows.
- Quality and cost effectiveness: Provides a high-quality, cost-effective option for purification processes.
- Broad antibody suitability: Supports purification of monoclonal, polyclonal, and engineered antibodies.
- Large-scale applicability: Is especially suited for large-scale purification of today’s and future therapeutic antibodies.
Product features of Protein A purification resins and buffers
- Affinity resin and buffer portfolio: Range of affinity resins and buffers for Protein A capture workflows.
- Workflow positioning: Capture-phase offering designed for antibody purification.
- Purification format: Affinity chromatography platform based on a highly specific interaction for separating antibodies from clarified feedstock.
- Process suitability: Recommended chemicals and buffers suitable for chromatography and purification processes.
- Purification focus: Configuration intended for high-throughput purification workflows.
How Protein A is used in purification capture
Protein A is used at the capture phase of purification to isolate antibodies from clarified feedstock, reduce process volume, and support downstream purification steps. Affinity chromatography enables this highly specific separation. The offering is suitable for monoclonal, polyclonal, and engineered antibodies, including large-scale therapeutic antibody purification.
- Equilibration: Used to prepare the column for Protein A capture.
- Middle wash No.2: Optional wash step used when additional cleanup is required.
Products listed for Protein A capture buffers
| Purpose | Buffers | Buffer pH | CV* | Product | Category | Compendial |
|---|---|---|---|---|---|---|
| Equilibration | PBS | Same as load sample | 5 | Sodium dihydrogen phosphate dihydrate | Emprove® Expert | Ph Eur, BP, USP, JPE |
| di-Sodium hydrogen phosphate dihydrate | Emprove® Expert | Ph Eur, BP, USP | ||||
| Sodium chloride | Emprove® Expert | Ph Eur, BP, ChP, JP, USP | ||||
| Middle wash No.1 (Choose equilibrium buffer) | PBS | Same as load sample | 5 | PBS (refer to ingredients above) | — | — |
| Middle wash No.2 (If necessary) | Option 1: Equilibration buffer and ≤ 0.5 M NaCl | Same as harvest sample | 5 | Sodium chloride | Emprove® Expert | Ph Eur, BP, ChP, JP, USP |
| PBS (refer to ingredients above) | — | — | ||||
| Option 2: Equilibration buffer with pH changed | 5.0~6.0 | 5 | Hydrochloric acid fuming 37% | Emprove® Expert | Ph Eur, BP, ChP, JP, NF | |
| PBS (refer to ingredients above) | — | — | ||||
| Option 3: Equilibration buffer with 0.1–1.0% polysorbate 80 | Same as harvest sample | 5 | Tween® 80 (Polysorbate) | Emprove® Essential | Ph Eur, JP, NF | |
| PBS (refer to ingredients above) | — | — | ||||
| Elution | Option 1: 0.1 M acetate buffer | 3.0~4.0 | 3 | Sodium acetate trihydrate | Emprove® Expert | Ph. Eur., BP, ChP, JP, USP |
| Acetic acid (glacial) 100% | Emprove® Expert | Ph Eur, BP, JP, USP | ||||
| Option 2: 0.1 M citrate buffer | 3.0~4.0 | 3 | Citric acid | Emprove® Expert | Ph Eur, BP, ChP, JP, USP, ACS | |
| tri-Sodium citrate dihydrate | Emprove® Expert | Ph Eur, BP, ChP, JP, USP, ACS, E 331 | ||||
| CIP** | Option 1: 150 mM phosphate acid | 1.5 | 3 | ortho-Phosphoric acid 85% | Emprove® Expert | Ph Eur, BP, ChP, JP, NF |
| Option 2: 0.1–0.3 NaOH | 13 | 3(~5) | Sodium hydroxide pellets | Emprove® Expert | Ph Eur, BP, ChP, JP, NF | |
| Column storage | pH 5.2±0.5 sodium acetate buffer with 1% benzyl alcohol | 5.2±0.5 | 3 | Sodium acetate trihydrate | Emprove® Expert | Ph. Eur., BP, ChP, JP, USP |
| Benzyl alcohol | Emprove® Expert | Ph Eur, BP, ChP, JP, NF | ||||
With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand. Easily order Protein A purification products and related bioprocessing chemicals, ensuring quality and compliance for every purification process.
Have questions about Products for Purification Processes: Protein A?
Simply fill out our Inquiry Form or chat with us instantly on Line using the buttons below.
We’re here to help!
Thai 
English