Pwo SuperYield DNA Polymerase (Roche) – High Fidelity, Maximum Yield for PCR Applications

Accurate DNA amplification is essential for molecular biology applications such as cloning and gene expression analysis, where researchers require both high yields and low error rates. Pwo SuperYield DNA Polymerase from Roche delivers outstanding fidelity and yield for demanding high fidelity PCR applications, while also helping to simplify experimental workflows.

Benefits and Key Features of Pwo SuperYield DNA Polymerase

• High yield with consistent high fidelity: Produces high yields of PCR product while maintaining consistent high fidelity for demanding amplification workflows.
• Reduced sequence errors: Helps minimize sequence errors, base exchanges, and frame shifts when combined with a proofreading reverse transcriptase.
• High fidelity without extensive optimization: Delivers approximately 18-fold higher fidelity compared to Taq DNA Polymerase without requiring extensive optimization.
• Performance with difficult templates: Supports amplification of GC-rich and complex DNA templates, helping improve yields where standard Taq-based PCR is challenging.
• Simplified cloning workflow: Reduces working steps in cloning by allowing enzyme digests directly in the Pwo SuperYield PCR mix.

Technical Features of Pwo SuperYield DNA Polymerase

• GC-RICH Solution: Compatible with a dedicated GC-RICH Solution to improve amplification of GC-rich DNA templates.
• PCR mix compatibility: Supports restriction enzyme digests performed directly in the Pwo SuperYield PCR mix to streamline cloning workflows.
• Reverse transcriptase pairing: Can be combined with a proofreading reverse transcriptase, such as the one used in the Transcriptor High Fidelity cDNA Synthesis Kit, for high fidelity RT-PCR.

Application and Usage of Pwo SuperYield DNA Polymerase in High Fidelity PCR

• High fidelity PCR: PCR assays where sequence accuracy and low error rates are critical.
• RT-PCR workflows: Used in combination with a proofreading reverse transcriptase, such as in the Transcriptor High Fidelity cDNA Synthesis Kit, when maximum fidelity is required to reduce sequence errors, base exchanges, and frame shifts in cDNA-derived PCR products.
• Cloning: Cloning workflows that depend on accurate DNA amplification prior to ligation or recombination.
• Site-directed mutagenesis: Mutagenesis protocols that require reliable incorporation of designed mutations.
• Gene expression analysis: Quantitative or qualitative gene expression studies where sequence integrity of amplicons is important.

Pwo SuperYield DNA Polymerase Selection

With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand, offering Pwo SuperYield DNA Polymerase and related PCR products. Rely on Chemical Express for authentic Roche and Merck high fidelity PCR solutions and benefit from trusted support and quality.