{"id":10406,"date":"2026-03-18T17:57:02","date_gmt":"2026-03-18T10:57:02","guid":{"rendered":"https:\/\/chemicalexpressth.com\/?p=10406"},"modified":"2026-08-11T15:39:57","modified_gmt":"2026-08-11T08:39:57","slug":"cellprime-rtransferrin-af-non-animal-transferrin-cell-culture-merck","status":"publish","type":"post","link":"https:\/\/chemicalexpressth.com\/th\/articles\/product-guide-articles\/cellprime-rtransferrin-af-non-animal-transferrin-cell-culture-merck\/","title":{"rendered":"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance"},"content":{"rendered":"<style>  .chx-standalone-article h1 {       color: #222 !important;       line-height: 1.2 !important;       font-weight: bold !important;       text-align: center !important;       margin-top: 40px !important;       margin-bottom: 30px !important;      text-transform: none !important;   }  .chx-standalone-article h2 {      color: #24326d !important;      font-weight: bold !important; 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}  }<\/style>\n<div class=\"chx-standalone-article\">\n<div data-elementor-type=\"wp-post\" data-elementor-id=\"10406\" class=\"elementor elementor-10406\" data-elementor-post-type=\"post\">\n<div class=\"elementor-element elementor-element-4cff914 e-flex e-con-boxed e-con e-parent\" data-id=\"4cff914\" data-element_type=\"container\" data-e-type=\"container\">\n<div class=\"e-con-inner\">\n<div class=\"elementor-element elementor-element-1c0edaa3 elementor-widget elementor-widget-text-editor\" data-id=\"1c0edaa3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n<div class=\"elementor-widget-container\">\n<h1>CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance<\/h1>\n<div style=\"margin-bottom: 32px; text-align: center;\"><img decoding=\"async\" class=\"lazyload\" style=\"width: 100%; max-width: 800px; height: auto; border-radius: 0px !important;\" src=\"data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\" data-src=\"https:\/\/chemicalexpressth.com\/wp-content\/uploads\/2026\/03\/CellPrime\u00ae-rTransferrin-AF-by-Merck-Superior-Non-Animal-Transferrin-for-Consistent-Cell-Culture-Performance.png\" alt=\"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance\" \/><noscript><img decoding=\"async\" class=\"lazyload lazyload\" style=\"width: 100%; max-width: 800px; height: auto; border-radius: 0px !important;\" src=\"data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\" data-src=\"https:\/\/chemicalexpressth.com\/wp-content\/uploads\/2026\/03\/CellPrime\u00ae-rTransferrin-AF-by-Merck-Superior-Non-Animal-Transferrin-for-Consistent-Cell-Culture-Performance.png\" alt=\"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance\" \/><noscript><img decoding=\"async\" class=\"lazyload lazyload lazyload\" style=\"width: 100%; max-width: 800px; height: auto; border-radius: 0px !important;\" src=\"data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\" data-src=\"https:\/\/chemicalexpressth.com\/wp-content\/uploads\/2026\/03\/CellPrime\u00ae-rTransferrin-AF-by-Merck-Superior-Non-Animal-Transferrin-for-Consistent-Cell-Culture-Performance.png\" alt=\"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance\" \/><noscript><img data-src=\"https:\/\/chemicalexpressth.com\/wp-content\/uploads\/2026\/03\/CellPrime\u00ae-rTransferrin-AF-by-Merck-Superior-Non-Animal-Transferrin-for-Consistent-Cell-Culture-Performance.png\" decoding=\"async\" class=\"lazyload lazyload lazyload\" style=\"width: 100%; max-width: 800px; height: auto; border-radius: 0px !important;\" src=\"data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\" alt=\"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance\" \/><noscript><img decoding=\"async\" class=\"lazyload lazyload lazyload\" style=\"width: 100%; max-width: 800px; height: auto; border-radius: 0px !important;\" src=\"https:\/\/chemicalexpressth.com\/wp-content\/uploads\/2026\/03\/CellPrime\u00ae-rTransferrin-AF-by-Merck-Superior-Non-Animal-Transferrin-for-Consistent-Cell-Culture-Performance.png\" alt=\"CellPrime\u00ae rTransferrin AF by Merck: Superior Non-Animal Transferrin for Consistent Cell Culture Performance\"><\/noscript><\/noscript><\/noscript><\/noscript><\/div>\n<p>Achieving reliable, high-performing cell cultures with CellPrime\u00ae rTransferrin AF requires eliminating variability and contamination risks from animal-derived proteins. CellPrime\u00ae rTransferrin AF delivers consistent iron transport and robust cell growth in serum-free media, and is designed to meet demanding industry standards. This non-animal, recombinant alternative supports optimal performance and facilitates regulatory compliance.<\/p>\n<h2>Benefits of CellPrime\u00ae rTransferrin AF for Serum-Free Cell Culture<\/h2>\n<h2>Benefits &amp; Key Features of CellPrime\u00ae rTransferrin AF<\/h2>\n<ul>\n<li><strong>Reduced variability in media formulations:<\/strong> Replacing serum transferrin with this recombinant supplement helps remove variability introduced by blood-derived proteins.<\/li>\n<li><strong>Lower contamination risk:<\/strong> Safeguards cell culture processes against adventitious virus or prion contamination associated with blood-derived proteins.<\/li>\n<li><strong>Optimized serum-free performance:<\/strong> Promotes viability and proliferation in cells grown in serum-free media by supporting optimal iron metabolism.<\/li>\n<li><strong>Comparable biological performance to serum transferrin:<\/strong> Several studies demonstrate similar potency in supporting cell growth and protein production.<\/li>\n<li><strong>Advantage over chemical iron supplements:<\/strong> Provides biological, not chemical, iron supplementation and has shown superior performance compared to chemical supplements across multiple cell lines.<\/li>\n<li><strong>Proven suitability for industrial cell culture:<\/strong> Recombinant transferrin expressed in <i>Saccharomyces cerevisiae<\/i>, specifically manufactured for industrial cell culture applications and used across multiple industrial cell lines.<\/li>\n<li><strong>Reliable lot-to-lot consistency:<\/strong> Consistently meets product specifications from batch to batch, supporting dependable process performance.<\/li>\n<li><strong>Regulatory-friendly, non-animal origin transferrin:<\/strong> Offers the advantages of plasma-derived transferrin while providing a non-animal origin, regulatory-friendly alternative for industrial cell culture applications.<\/li>\n<li><strong>cGMP manufacturing and convenient format:<\/strong> Manufactured under cGMP in FDA-inspected facilities and supplied as a filtered liquid that can be directly added to culture medium, often in combination with recombinant albumin and recombinant insulin in media formulations.<\/li>\n<\/ul>\n<h2>Features of CellPrime\u00ae rTransferrin AF<\/h2>\n<ul>\n<li><strong>Product Classification<\/strong>: Recombinant iron-transport protein (rTransferrin) expressed as a non-animal origin (NAO) analog to plasma-derived transferrin.<\/li>\n<li><strong>Expression System<\/strong>: Protein synthesized in Saccharomyces cerevisiae utilizing a recombinant yeast platform.<\/li>\n<li><strong>Manufacturing Standard<\/strong>: cGMP-compliant production within FDA-inspected industrial facilities.<\/li>\n<li><strong>Functional Mechanism<\/strong>: Biochemical framework designed for extracellular iron binding and transport within cell culture systems.<\/li>\n<li><strong>Supplementation Format<\/strong>: Biological iron delivery architecture utilized in combination with recombinant albumin and insulin media supplements.<\/li>\n<li><strong>Physical State<\/strong>: Sterility-filtered liquid formulation configured for direct integration into culture media.<\/li>\n<li><strong>Regulatory Profile<\/strong>: Non-animal origin composition structured for alignment with industrial bioprocessing compliance standards.<\/li>\n<\/ul>\n<h2>Technical Specifications of CellPrime\u00ae rTransferrin AF<\/h2>\n<ul>\n<li><strong>Protein by Kjeldahl:<\/strong> 18-22 g\/L<\/li>\n<li><strong>Purity by RP-HPLC:<\/strong> \u226595%<\/li>\n<li><strong>pH (1% solution) by standard method:<\/strong> 6.0-8.0<\/li>\n<li><strong>Inspection:<\/strong> Clear, red\/pink solution<\/li>\n<li><strong>Endotoxin by LAL:<\/strong> \u226420 EU\/mL<\/li>\n<li><strong>Iron content by atomic absorption:<\/strong> 1.3-1.7 mg\/g<\/li>\n<li><strong>Total iron capacity by atomic absorption:<\/strong> \u2265 1.0 mg\/g<\/li>\n<\/ul>\n<h2>Application and Usage of CellPrime\u00ae rTransferrin AF<\/h2>\n<ul>\n<li><strong>Serum-free cell culture use:<\/strong> CellPrime\u00ae rTransferrin AF is used in cell culture systems to facilitate extracellular iron binding and transport, supporting iron metabolism in cells grown in serum-free media.<\/li>\n<li><strong>Media formulation component:<\/strong> CellPrime\u00ae rTransferrin AF is often used to replace serum transferrin in combination with recombinant albumin and recombinant insulin as a supplement mix in cell culture media formulations.<\/li>\n<li><strong>Industrial cell culture applications:<\/strong> Suitable for use in upstream industrial cell culture processes across multiple cell lines.<\/li>\n<li><strong>Direct medium addition:<\/strong> CellPrime\u00ae rTransferrin AF is supplied as a 0.2 \u03bcm filtered liquid formulated in 145 mM NaCl, pH 6.8, at 20 g\/L and can be directly added to the culture medium.<\/li>\n<li><strong>Recommended use concentration:<\/strong> A titration should be performed for each application because the optimum concentration may vary by cell type, culture conditions, and medium composition, with a recommended final concentration range of 1 to 10 mg\/L.<\/li>\n<li><strong>Substitution in existing workflows:<\/strong> CellPrime\u00ae rTransferrin AF can be directly substituted for bovine or human-derived transferrin.<\/li>\n<li><strong>Filtration guidance:<\/strong> If additional filtration is required in your process, use a low protein-binding membrane such as polyvinylidene difluoride, polyethersulfone, or cellulose acetate with a pore size of 0.22 \u03bcm.<\/li>\n<li><strong>Adaptation consideration:<\/strong> Cultures previously maintained in media containing iron chelators may require an adaptation phase, and the full effects of CellPrime\u00ae rTransferrin AF may not be apparent until after one or more sub-culturing steps.<\/li>\n<\/ul>\n<h2>Products Available Under CellPrime\u00ae rTransferrin AF<\/h2>\n<div class=\"res-table-wrapper\">\n<table>\n<thead>\n<tr>\n<th style=\"background-color: #24326d; color: #ffffff;\"><strong>Description<\/strong><\/th>\n<th style=\"background-color: #24326d; color: #ffffff;\"><strong>Qty\/Pk<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>CellPrime\u00ae rTransferrin AF 2% solution<\/td>\n<td>0.2 g\/10 mL<\/td>\n<\/tr>\n<tr>\n<td>CellPrime\u00ae rTransferrin AF 2% solution<\/td>\n<td>1 g\/50 mL<\/td>\n<\/tr>\n<tr>\n<td>CellPrime\u00ae rTransferrin AF 2% solution<\/td>\n<td>Bulk<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>With over 23 years of expertise, Chemical Express is the official distributor of Merck in Thailand. Access CellPrime\u00ae rTransferrin AF and a full range of Merck cell culture supplements through Chemical Express, ensuring trusted quality and regulatory compliance for your applications.<\/p>\n<div style=\"text-align: center; margin-top: 64px; padding-bottom: 60px;\">\n<h3 style=\"margin-bottom: 8px;\">Need technical guidance or ready to order CellPrime\u00ae rTransferrin AF?<\/h3>\n<p>Simply fill out our Inquiry Form or chat with us instantly on Line using the buttons below.<br \/>We&#8217;re here to help!<\/p>\n<div style=\"display: flex; justify-content: center; align-items: center; gap: 16px; flex-wrap: wrap; margin-bottom: 20px;\"><a style=\"display: inline-flex; align-items: center; justify-content: center; padding: 14px 28px; background: #0b5ed7; color: #ffffff; text-decoration: none; border-radius: 8px; font-weight: 600; font-size: 16px; line-height: 1; white-space: nowrap;\" 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