Cell Culture
Cell culture plays an important role in biology, life sciences and pharmacological research and manufacturing. Our Cell Culture & Analysis products ensure that you have the tools needed to drive breakthroughs in cell biology, drug discovery, regenerative medicine and so on.
From basic cell culture essentials to specialized formulations, our products support a wide array of cell types and applications to meet the diverse needs of researchers and professionals. Whether you are performing routine cell counting, cell imaging, or advanced cellular assays, our products deliver unparalleled performance and consistency.
Cell Culture Media & Buffers
Whether you’re cultivating adherent or suspension phenotypes, with or without FBS, and prefer high- or low-glutamine options, we have you covered. Our ready-to-use liquid and easy-to-store powder formulations cater to your convenience. And rest assured, our media and salts are meticulously qualified for cell culture applications and manufactured in cutting-edge facilities.
- Bioprocessing Cell Culture Media
- Classical Media & Buffers
- Plant Culture Media
Cell Lines & Specialty Cell Culture
Depending on your research, you’ll need the specific cell lines or primary cells of interest. Our curated selection ensures reliable and high-quality options, allowing you to advance your experiments with confidence.
- Stem Cells
- Mouse Embryo Media
- Primary Cells
- Mammalian Cell Lines
- Bioprocessing Catalog Cell Lines
3D Cell Culture
Designed to mimic the in vivo environment, our 3D Cell Culture products provide great physiological relevance, fostering more accurate cell behavior and responses. Our user-friendly products ensure high cell viability, enhanced physiological relevance, and accurate representation of cell behavior.
- Hydrogels
- Scaffolds
- Organoids
Sterile Lab Media Filtration & Cultureware
Sterile laboratory media filters play a vital role in preventing contamination in cultures. Designed to eliminate particulate contamination, sterilize liquid reagents, and clarify solutions, our filters come in various formats like syringe filters, vacuum-driven filter units, and bottle top filters. Featuring high-performance membranes, our filtration solutions ensure the integrity of laboratory reagents, buffers, and media.
- Sterile Laboratory Filtration Devices
- Vacuum Filtration
- Filter Plates
- Syringe Filters
- Cell Culture Supplies
- Cell Culture Inserts & Plates
Cell Culture Supplements & Reagents
Ensuring adequate cell growth relies on many culture conditions, including the correct media formulation for your cell line. Tailored for diverse cell types, our high-quality supplements and reagents enhance viability, proliferation, and functionality. From serum alternatives to specialized growth factors, ensure reliable results for your research and bioprocessing needs.
- Cell Freezing Reagents
- Plant Culture Reagents
- Cell Dissociation Reagents
- Albumins and Transport Proteins
- Hormones
- Supplements for Cell Culture
- Sera
- Human Serum
- Fetal Bovine Serum (FBS)
- Attachment Factors
- Amino Acids
- Antibiotics
- Carbohydrates
- Vitamins
- Growth Factors and Cytokines
Cell Analysis
Various cell analysis techniques are employed to understand factors influencing cell proliferation, death, health, and function. Explore a spectrum of cell analysis techniques with our instruments and reagents. From live cell imaging to cell counting, health assessment, isolation, and migration studies, our tools empower researchers. In addition to standard kits, our specialty instruments offer extra dimensions for in-depth cell process investigations.
- Live Cell Imaging Reagents
- Cell Health Analysis
- Cell Migration & Invasion Assays
- Cell Isolation Kits
- Cell Counting Equipment
- Cell Imaging Instruments
Animal Component Free Cell Culture Reagents
Explore our Animal Component Free Cell Culture Reagents—ideal for researchers seeking consistent and ethical alternatives. Our reagents, free from animal-derived ingredients, ensure optimal cell growth with minimal lot-to-lot variations. Our high-quality animal component-free (ACF), non-animal origin (NAO) and serum-free cell culture media, supplements, reagents, and growth factors have high biological activity. Compliant with high-quality and sustainability standards, they’re perfect for applications like stem cell culture, inflammation studies, cancer cell culture, and antibody/vaccine development.
- Animal origin-free & serum-free media
- Animal-free cell dissociation reagents
- Chemically defined supplements
- Animal component-free growth factors & Cytokines
- Animal-free cell freezing media
Discover an extensive collection of Cell Culture & Analysis products we offer from Sigma Aldrich through our Search Tool.
For other brands, please contact us.
| Section | Name | Cat.No. |
|---|---|---|
| Serum | Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma | F2442 |
| Serum | Fetal Bovine Serum, Heat Inactivated, USA origin, sterile-filtered, suitable for cell culture | F4135 |
| Serum | Fetal Bovine Serum, USDA approved, non-USA origin, sterile-filtered, suitable for cell culture | F0926 |
| Serum | Fetal Bovine Serum, USDA approved, non-USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture | 12306C |
| Serum | Fetal Bovine Serum, Non-USA origin, sterile-filtered, suitable for cell culture | F7524 |
| Serum | Fetal Bovine Serum, Heat Inactivated, Non-USA origin, sterile-filtered, suitable for cell culture | F9665 |
| Amino Acids | L-Glutamine solution, 200mM, solution, sterile-filtered, suitable for cell culture | G7513 |
| Amino Acids | MEM Non-essential Amino Acid Solution (100×), liquid, without L-glutamine, sterile-filtered, suitable for cell culture | M7145 |
| Antibiotics | Amphotericin B solution, sterile-filtered, 250 μg/mL in deionized water, suitable for cell culture | A2942 |
| Antibiotics | Amphotericin B solubilized, powder, γ-irradiated, suitable for cell culture | A9528 |
| Antibiotics | Ampicillin sodium salt, suitable for cell culture, 91.0–100.5% (titration), powder | A0166 |
| Antibiotics | Antibiotic-Antimycotic Stabilized, suspension, stabilized, sterile-filtered, suitable for cell culture | A5955 |
| Antibiotics | G418 disulfate salt solution, 50mg/mL solution, sterile-filtered, suitable for cell culture | G8168 |
| Antibiotics | Gelatin solution, 2% in water, tissue culture grade, sterile, Type B, suitable for cell culture | G1393 |
| Antibiotics | Gentamicin solution, 50mg/mL in deionized water, liquid, sterile-filtered, suitable for cell culture | G1397 |
| Antibiotics | Gentamicin solution, 10mg/mL in deionized water, liquid, sterile-filtered, suitable for cell culture | G1272 |
| Antibiotics | Hygromycin B from Streptomyces hygroscopicus, powder, suitable for cell culture, suitable for insect cell culture | H3274 |
| Antibiotics | Mitomycin C from Streptomyces caespitosus, powder, suitable for cell culture | M4287 |
| Antibiotics | Neomycin solution, liquid, sterile-filtered, suitable for cell culture | N1142 |
| Antibiotics | Nystatin preparation, suspension, sterile; aseptically processed, suitable for cell culture | N1638 |
| Antibiotics | Penicillin-Streptomycin, 100×, liquid, sterile-filtered, suitable for cell culture | P0781 |
| Antibiotics | Penicillin-Streptomycin, 50×, liquid, stabilized, sterile-filtered, suitable for cell culture | P4458 |
| Antibiotics | Penicillin-Streptomycin, 100×, liquid, stabilized, sterile-filtered, suitable for cell culture | P4333 |
| Antibiotics | Puromycin dihydrochloride from Streptomyces alboniger, powder, ≥98% (TLC), suitable for cell culture | P8833 |
| Attachment Factors | Fibronectin from bovine plasma, solution, 1mg/mL (in 0.5M NaCl, 0.05M Tris, pH 7.5), suitable for cell culture, sterile | F1141 |
| Attachment Factors | Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1mg/mL in Tris buffered NaCl, sterile-filtered, suitable for cell culture | L2020 |
| Attachment Factors | Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, γ-irradiated, lyophilized powder, suitable for cell culture | P7280 |
| Attachment Factors | Poly-D-lysine hydrobromide, mol wt 70,000-150,000, γ-irradiated, lyophilized powder, suitable for cell culture | P6407 |
| Attachment Factors | Poly-D-lysine hydrobromide, mol wt >300,000, γ-irradiated, lyophilized powder, suitable for cell culture | P7405 |
| Attachment Factors | Poly-L-lysine hydrobromide, mol wt 70,000-150,000, γ-irradiated, lyophilized powder, suitable for cell culture | P6282 |
| Attachment Factors | Poly-L-lysine solution, 0.01%, mol wt 70,000-150,000, sterile-filtered, suitable for cell culture | P4707 |
| Attachment Factors | Poly-L-lysine solution, 0.01%, mol wt 150,000-300,000, sterile-filtered, suitable for cell culture | P4832 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline, With MgCl_{2} and CaCl_{2}, liquid, sterile-filtered, suitable for cell culture | D8662 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, sterile-filtered, liquid, suitable for cell culture | D8537 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline, Without calcium chloride, powder, suitable for cell culture | D5773 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline, Modified, Without calcium chloride and magnesium chloride, powder, suitable for cell culture | D5652 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline 10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | D1408 |
| Balanced Salts | Dulbecco's Phosphate Buffered Saline, Modified, with 1,000mg/L glucose, sodium pyruvate, streptomycin sulfate, kanamycin monosulfate, CaCl_{2}, MgCl_{2}, liquid, sterile-filtered, suitable for cell culture | D4031 |
| Balanced Salts | Earle's Balanced Salts, With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | E2888 |
| Balanced Salts | Hanks' Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | H6648 |
| Balanced Salts | Hanks' Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture | H8264 |
| Balanced Salts | Hanks' Balanced Salt solution, With sodium bicarbonate, sterile-filtered, suitable for cell culture | H9269 |
| Balanced Salts | Hanks' Balanced Salt solution, Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | H9394 |
| Balanced Salts | Krebs-Henseleit Buffer Modified, With 2000mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture | K3753 |
| Biochemicals | Heparin sodium salt from porcine intestinal mucosa, Grade I-A, suitable for cell culture, activity: ≥140 USP units/mg, powder | H3149 |
| Biological Buffers | HEPES solution, 1M, pH 7.0-7.6, sterile-filtered, suitable for cell culture | H0887 |
| Biological Buffers | Sodium bicarbonate, powder, ≥99.5%, suitable for cell culture, suitable for insect cell culture | S5761 |
| Biological Buffers | Sodium bicarbonate solution, solution (7.5%), sterile-filtered, suitable for cell culture | S8761 |
| Biological Buffers | Sodium chloride solution (0.9%), sterile-filtered, suitable for cell culture | S8776 |
| Cell Dissociation | Cell Dissociation Solution Non-enzymatic 1×, Prepared in Hanks' Balanced Salt Solution without calcium and magnesium, sterile-filtered, suitable for cell culture | C5789 |
| Cell Dissociation | Cell Dissociation Solution Non-enzymatic 1×, Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, suitable for cell culture | C5914 |
| Cell Dissociation | Trypsin-EDTA solution, 0.25%, 2.5g porcine trypsin and 0.2g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red, sterile-filtered, suitable for cell culture | T4049 |
| Cell Dissociation | Trypsin-EDTA solution, 1×, 0.5g porcine trypsin and 0.2g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red, sterile-filtered, suitable for cell culture | T3924 |
| Cell Dissociation | Trypsin-EDTA solution, 10×, 5.0g porcine trypsin and 2g EDTA • 4Na per liter of 0.9% sodium chloride, sterile-filtered, suitable for cell culture | T4174 |
| Growth Factors | Endothelial cell growth supplement from bovine neural tissue, suitable for cell culture | E2759 |
| Growth Factors | Epidermal Growth Factor human, recombinant, expressed in Escherichia coli, lyophilized powder, suitable for cell culture | E9644 |
| Growth Factors | Fibroblast Growth Factor-Basic human, recombinant, expressed in Escherichia coli, suitable for cell culture | F0291 |
| Growth Factors | Insulin solution from bovine pancreas, 10mg/mL insulin in 25mM HEPES, pH 8.2, sterile-filtered, suitable for cell culture | I0516 |
| Hormones | Gonadotropin from pregnant mare serum, lyophilized powder, suitable for mouse embryo cell culture | G4527 |
| Hormones | Insulin from bovine pancreas, powder, suitable for cell culture | I6634 |
| Hormones | Insulin from bovine pancreas, lyophilized powder, activity: ≥25 USP units/mg, secondary activity: 2500 units (HPLC, /100mg vial), γ-irradiated, suitable for cell culture | I1882 |
| Hormones | Insulin solution human, Chemically defined, recombinant from Saccharomyces cerevisiae, solution, sterile-filtered, suitable for cell culture | I9278 |
| Hormones & Misc.Reagents | ITS Liquid Media Supplement (100×), liquid, sterile-filtered, suitable for cell culture | I3146 |
| Hormones & Misc.Reagents | OPI Media Supplement, Hybri-Max™, γ-irradiated, lyophilized powder, suitable for hybridoma | O5003 |
| Hormones & Misc.Reagents | Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma | R7757 |
| Hybridoma | HAT Media Supplement (50×) Hybri-Max™, lyophilized powder, γ-irradiated, suitable for hybridoma | H0262 |
| Hybridoma | Histopaque®-1077 Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma | H8889 |
| Lectins | Concanavalin A from Canavalia ensiformis (Jackbean), Type IV-S, lyophilized powder, aseptically processed, suitable for cell culture | C5275 |
| Lipopolysaccharides | Lipopolysaccharides from Escherichia coli 0111:B4, suitable for cell culture, γ-irradiated | L4391 |
| Lipopolysaccharides | Lipopolysaccharides from Escherichia coli 055:B5, suitable for cell culture, γ-irradiated | L6529 |
| Lipopolysaccharides | Lipopolysaccharides from Escherichia coli 026:B6, suitable for cell culture, γ-irradiated | L2654 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture | D5796 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma | D5671 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | D6429 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | D6546 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture | D1145 |
| Media | Dulbecco's Modified Eagle's Medium – high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate. Use at 13.4 g/L., powder, suitable for cell culture | D5648 |
| Media | Dulbecco's Modified Eagle's Medium-low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | D6046 |
| Media | Dulbecco's Modified Eagle's Medium-low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | D5546 |
| Media | Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | D6421 |
| Media | Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | G5154 |
| Media | Iscove's Modified Dulbecco's Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma | I3390 |
| Media | L-15 Medium (Leibovitz), Without L-glutamine., liquid, sterile-filtered, suitable for cell culture | L5520 |
| Media | Medium 199, With Earle's salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | M4530 |
| Media | Minimum Essential Medium Eagle, HEPES Modification, with Earle's salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | M7278 |
| Media | Minimum Essential Medium Eagle, Joklik Modification, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | M8028 |
| Media | Minimum Essential Medium Eagle, Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture | M0518 |
| Media | Minimum Essential Medium Eagle, With Earle's salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | M4655 |
| Media | Minimum Essential Medium Eagle, With Earle's salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | M2279 |
| Media | Minimum Essential Medium Eagle, With Earle's salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | M5650 |
| Media | Nutrient Mixture F-10 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | N6013 |
| Media | RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | R8758 |
| Media | RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture | R5886 |
| Media | RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | R0883 |
| Media | RPMI-1640 Medium, Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture | R7509 |
| Miscellaneous Reagents | Sodium Hydroxide Solution, 1.0N, suitable for cell culture | S2770 |
| Miscellaneous Reagents | Sodium Pyruvate Solution, 100mM, sterile-filtered, suitable for cell culture | S8636 |
| Miscellaneous Reagents | Water, sterile-filtered, suitable for cell culture | W3500 |
| Reagents | Albumin from Bovine Serum, powder, Fatty Acid-free, suitable for cell culture, ≥96% (agarose gel electrophoresis) | A8806 |
| Reagents | Cell Freezing Medium - DMSO 1×, sterile-filtered, suitable for cell culture | C6164 |
| Reagents & Biochemicals | Linoleic Acid - Albumin from Bovine Serum Albumin, liquid, sterile-filtered, suitable for cell culture | L9530 |
| Reagents & Hybridoma | Dimethyl Sulfoxide, liquid (temperature-dependent), ≥99.7%, Hybri-Max™, sterile-filtered, suitable for hybridoma | D2650 |
| Reagents & Hybridoma | Dimethyl Sulfoxide, Biotechnology Performance Certified, sterile-filtered, meets EP, USP testing specifications, suitable for hybridoma | D2438 |
| Stains, Dyes & Indicators | Thiazolyl Blue Tetrazolium Bromide, powder, suitable for cell culture, suitable for insect cell culture | M5655 |
| Stains, Dyes & Indicators | Trypan Blue Solution, 0.4%, liquid, sterile-filtered, suitable for cell culture | T8154 |
| Supplements | D-(+)-Glucose Solution, 45% in water, tissue culture grade, autoclaved, suitable for cell culture | G8769 |
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